Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19520 and BPHYT_RS19525 are separated by 94 nucleotides BPHYT_RS19525 and BPHYT_RS19530 are separated by 105 nucleotides BPHYT_RS19530 and BPHYT_RS19535 are separated by 239 nucleotides
BPHYT_RS19520: BPHYT_RS19520 - phenylalanine 4-monooxygenase, at 4,392,660 to 4,393,550
_RS19520
BPHYT_RS19525: BPHYT_RS19525 - pterin-4-alpha-carbinolamine dehydratase, at 4,393,645 to 4,393,935
_RS19525
BPHYT_RS19530: BPHYT_RS19530 - hypothetical protein, at 4,394,041 to 4,394,265
_RS19530
BPHYT_RS19535: BPHYT_RS19535 - XRE family transcriptional regulator, at 4,394,505 to 4,395,191
_RS19535
Position (kb) 4393
4394 Strain fitness (log2 ratio) -1
0
1
2 at 4392.753 kb on + strand, within BPHYT_RS19520 at 4392.789 kb on + strand, within BPHYT_RS19520 at 4393.035 kb on + strand, within BPHYT_RS19520 at 4393.110 kb on + strand, within BPHYT_RS19520 at 4393.110 kb on + strand, within BPHYT_RS19520 at 4393.110 kb on + strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.123 kb on - strand, within BPHYT_RS19520 at 4393.246 kb on - strand, within BPHYT_RS19520 at 4393.285 kb on - strand, within BPHYT_RS19520 at 4393.405 kb on + strand, within BPHYT_RS19520 at 4393.545 kb on - strand at 4393.837 kb on + strand, within BPHYT_RS19525 at 4393.924 kb on - strand at 4393.924 kb on - strand at 4393.934 kb on - strand at 4393.947 kb on + strand at 4393.948 kb on - strand at 4393.948 kb on - strand at 4394.017 kb on - strand at 4394.049 kb on + strand at 4394.050 kb on - strand at 4394.066 kb on + strand, within BPHYT_RS19530 at 4394.066 kb on + strand, within BPHYT_RS19530 at 4394.067 kb on - strand, within BPHYT_RS19530 at 4394.067 kb on - strand, within BPHYT_RS19530 at 4394.131 kb on + strand, within BPHYT_RS19530 at 4394.132 kb on - strand, within BPHYT_RS19530 at 4394.148 kb on - strand, within BPHYT_RS19530 at 4394.479 kb on + strand at 4394.489 kb on + strand at 4394.796 kb on + strand, within BPHYT_RS19535 at 4394.797 kb on - strand, within BPHYT_RS19535 at 4394.797 kb on - strand, within BPHYT_RS19535
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 4,392,753 + BPHYT_RS19520 0.10 +2.1 4,392,789 + BPHYT_RS19520 0.14 +1.0 4,393,035 + BPHYT_RS19520 0.42 +1.0 4,393,110 + BPHYT_RS19520 0.51 +0.1 4,393,110 + BPHYT_RS19520 0.51 +0.3 4,393,110 + BPHYT_RS19520 0.51 -1.6 4,393,111 - BPHYT_RS19520 0.51 -0.2 4,393,111 - BPHYT_RS19520 0.51 -0.6 4,393,111 - BPHYT_RS19520 0.51 +0.9 4,393,111 - BPHYT_RS19520 0.51 -0.5 4,393,111 - BPHYT_RS19520 0.51 +0.1 4,393,123 - BPHYT_RS19520 0.52 -1.0 4,393,246 - BPHYT_RS19520 0.66 -1.2 4,393,285 - BPHYT_RS19520 0.70 +0.0 4,393,405 + BPHYT_RS19520 0.84 -1.6 4,393,545 - +0.7 4,393,837 + BPHYT_RS19525 0.66 +0.7 4,393,924 - -0.2 4,393,924 - -0.5 4,393,934 - +0.8 4,393,947 + -0.3 4,393,948 - -0.8 4,393,948 - +0.1 4,394,017 - -0.0 4,394,049 + +2.3 4,394,050 - +0.2 4,394,066 + BPHYT_RS19530 0.11 -0.4 4,394,066 + BPHYT_RS19530 0.11 -0.3 4,394,067 - BPHYT_RS19530 0.12 -0.9 4,394,067 - BPHYT_RS19530 0.12 +1.6 4,394,131 + BPHYT_RS19530 0.40 +0.7 4,394,132 - BPHYT_RS19530 0.40 -1.0 4,394,148 - BPHYT_RS19530 0.48 +0.3 4,394,479 + -0.3 4,394,489 + -0.0 4,394,796 + BPHYT_RS19535 0.42 -0.2 4,394,797 - BPHYT_RS19535 0.43 +0.4 4,394,797 - BPHYT_RS19535 0.43 +0.5
Or see this region's nucleotide sequence