Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10925

Experiment: 24hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10915 and BPHYT_RS10920 are separated by 331 nucleotidesBPHYT_RS10920 and BPHYT_RS10925 are separated by 321 nucleotidesBPHYT_RS10925 and BPHYT_RS10930 are separated by 86 nucleotidesBPHYT_RS10930 and BPHYT_RS10935 are separated by 67 nucleotides BPHYT_RS10915: BPHYT_RS10915 - radical SAM protein, at 2,484,196 to 2,485,617 _RS10915 BPHYT_RS10920: BPHYT_RS10920 - cold-shock protein, at 2,485,949 to 2,486,152 _RS10920 BPHYT_RS10925: BPHYT_RS10925 - aldehyde dehydrogenase, at 2,486,474 to 2,487,919 _RS10925 BPHYT_RS10930: BPHYT_RS10930 - GCN5 family N-acetyltransferase, at 2,488,006 to 2,488,605 _RS10930 BPHYT_RS10935: BPHYT_RS10935 - 2-keto-3-deoxygluconate permease, at 2,488,673 to 2,489,698 _RS10935 Position (kb) 2486 2487 2488Strain fitness (log2 ratio) -3 -2 -1 0 1at 2485.678 kb on + strandat 2485.679 kb on - strandat 2485.711 kb on + strandat 2487.716 kb on + strand, within BPHYT_RS10925at 2487.717 kb on - strand, within BPHYT_RS10925at 2487.881 kb on + strandat 2487.917 kb on + strandat 2488.175 kb on - strand, within BPHYT_RS10930at 2488.353 kb on + strand, within BPHYT_RS10930at 2488.366 kb on + strand, within BPHYT_RS10930at 2488.542 kb on + strand, within BPHYT_RS10930at 2488.543 kb on - strand, within BPHYT_RS10930at 2488.635 kb on + strandat 2488.639 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10
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2,485,678 + +1.4
2,485,679 - +0.2
2,485,711 + -1.6
2,487,716 + BPHYT_RS10925 0.86 +1.0
2,487,717 - BPHYT_RS10925 0.86 -0.0
2,487,881 + -0.6
2,487,917 + +0.7
2,488,175 - BPHYT_RS10930 0.28 +1.7
2,488,353 + BPHYT_RS10930 0.58 -2.6
2,488,366 + BPHYT_RS10930 0.60 -2.8
2,488,542 + BPHYT_RS10930 0.89 -1.9
2,488,543 - BPHYT_RS10930 0.90 +1.5
2,488,635 + -0.6
2,488,639 + -1.9

Or see this region's nucleotide sequence