Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10910 and BPHYT_RS10915 overlap by 4 nucleotides BPHYT_RS10915 and BPHYT_RS10920 are separated by 331 nucleotides BPHYT_RS10920 and BPHYT_RS10925 are separated by 321 nucleotides
BPHYT_RS10910: BPHYT_RS10910 - glucosyltransferase, at 2,483,039 to 2,484,199
_RS10910
BPHYT_RS10915: BPHYT_RS10915 - radical SAM protein, at 2,484,196 to 2,485,617
_RS10915
BPHYT_RS10920: BPHYT_RS10920 - cold-shock protein, at 2,485,949 to 2,486,152
_RS10920
BPHYT_RS10925: BPHYT_RS10925 - aldehyde dehydrogenase, at 2,486,474 to 2,487,919
_RS10925
Position (kb)
2484
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2483.698 kb on + strand, within BPHYT_RS10910 at 2483.748 kb on + strand, within BPHYT_RS10910 at 2483.748 kb on + strand, within BPHYT_RS10910 at 2483.977 kb on - strand, within BPHYT_RS10910 at 2484.163 kb on - strand at 2484.329 kb on + strand at 2484.330 kb on - strand at 2484.330 kb on - strand at 2484.947 kb on + strand, within BPHYT_RS10915 at 2485.028 kb on + strand, within BPHYT_RS10915 at 2485.029 kb on - strand, within BPHYT_RS10915 at 2485.413 kb on - strand, within BPHYT_RS10915 at 2485.678 kb on + strand at 2485.679 kb on - strand at 2485.711 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 2,483,698 + BPHYT_RS10910 0.57 +0.5 2,483,748 + BPHYT_RS10910 0.61 +0.2 2,483,748 + BPHYT_RS10910 0.61 -1.3 2,483,977 - BPHYT_RS10910 0.81 +1.4 2,484,163 - +1.9 2,484,329 + +0.1 2,484,330 - -1.1 2,484,330 - -0.4 2,484,947 + BPHYT_RS10915 0.53 -1.0 2,485,028 + BPHYT_RS10915 0.59 -0.0 2,485,029 - BPHYT_RS10915 0.59 -2.6 2,485,413 - BPHYT_RS10915 0.86 -1.0 2,485,678 + +1.4 2,485,679 - +0.2 2,485,711 + -1.6
Or see this region's nucleotide sequence