Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10205 and BPHYT_RS10210 are separated by 151 nucleotides BPHYT_RS10210 and BPHYT_RS10215 are separated by 88 nucleotides BPHYT_RS10215 and BPHYT_RS10220 are separated by 166 nucleotides BPHYT_RS10220 and BPHYT_RS10225 are separated by 62 nucleotides
BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344
_RS10205
BPHYT_RS10210: BPHYT_RS10210 - LysR family transcriptional regulator, at 2,325,496 to 2,326,395
_RS10210
BPHYT_RS10215: BPHYT_RS10215 - sulfate transporter, at 2,326,484 to 2,326,954
_RS10215
BPHYT_RS10220: BPHYT_RS10220 - hypothetical protein, at 2,327,121 to 2,327,330
_RS10220
BPHYT_RS10225: BPHYT_RS10225 - GYD family protein, at 2,327,393 to 2,327,686
_RS10225
Position (kb) 2325
2326
2327 Strain fitness (log2 ratio) -1
0
1
2 at 2324.721 kb on - strand, within BPHYT_RS10205 at 2324.814 kb on - strand, within BPHYT_RS10205 at 2325.050 kb on - strand, within BPHYT_RS10205 at 2325.050 kb on - strand, within BPHYT_RS10205 at 2325.591 kb on - strand, within BPHYT_RS10210 at 2326.696 kb on + strand, within BPHYT_RS10215 at 2327.247 kb on - strand, within BPHYT_RS10220
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 2,324,721 - BPHYT_RS10205 0.64 +1.2 2,324,814 - BPHYT_RS10205 0.69 +2.6 2,325,050 - BPHYT_RS10205 0.83 +0.3 2,325,050 - BPHYT_RS10205 0.83 +0.4 2,325,591 - BPHYT_RS10210 0.11 -1.1 2,326,696 + BPHYT_RS10215 0.45 -0.3 2,327,247 - BPHYT_RS10220 0.60 +2.3
Or see this region's nucleotide sequence