Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09535 and BPHYT_RS09540 are separated by 1 nucleotides BPHYT_RS09540 and BPHYT_RS09545 are separated by 163 nucleotides BPHYT_RS09545 and BPHYT_RS09550 are separated by 130 nucleotides
BPHYT_RS09535: BPHYT_RS09535 - BolA family transcriptional regulator, at 2,163,870 to 2,164,190
_RS09535
BPHYT_RS09540: BPHYT_RS09540 - intracellular septation protein A, at 2,164,192 to 2,164,722
_RS09540
BPHYT_RS09545: BPHYT_RS09545 - peptide methionine sulfoxide reductase, at 2,164,886 to 2,165,308
_RS09545
BPHYT_RS09550: BPHYT_RS09550 - hypothetical protein, at 2,165,439 to 2,166,995
_RS09550
Position (kb)
2164
2165
2166 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2163.905 kb on - strand, within BPHYT_RS09535 at 2163.961 kb on + strand, within BPHYT_RS09535 at 2163.961 kb on + strand, within BPHYT_RS09535 at 2163.962 kb on - strand, within BPHYT_RS09535 at 2164.171 kb on - strand at 2164.171 kb on - strand at 2164.207 kb on - strand at 2164.888 kb on - strand at 2164.904 kb on + strand at 2164.962 kb on - strand, within BPHYT_RS09545 at 2165.145 kb on + strand, within BPHYT_RS09545 at 2165.221 kb on - strand, within BPHYT_RS09545 at 2165.404 kb on - strand at 2165.494 kb on + strand at 2165.494 kb on + strand at 2165.617 kb on - strand, within BPHYT_RS09550 at 2166.077 kb on - strand, within BPHYT_RS09550 at 2166.082 kb on + strand, within BPHYT_RS09550 at 2166.182 kb on + strand, within BPHYT_RS09550 at 2166.183 kb on - strand, within BPHYT_RS09550 at 2166.183 kb on - strand, within BPHYT_RS09550 at 2166.183 kb on - strand, within BPHYT_RS09550
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 2,163,905 - BPHYT_RS09535 0.11 -0.3 2,163,961 + BPHYT_RS09535 0.28 -0.3 2,163,961 + BPHYT_RS09535 0.28 +1.3 2,163,962 - BPHYT_RS09535 0.29 +0.1 2,164,171 - -1.1 2,164,171 - -0.8 2,164,207 - -0.3 2,164,888 - +1.5 2,164,904 + -0.3 2,164,962 - BPHYT_RS09545 0.18 +1.3 2,165,145 + BPHYT_RS09545 0.61 +0.7 2,165,221 - BPHYT_RS09545 0.79 -2.1 2,165,404 - -0.5 2,165,494 + +2.6 2,165,494 + -0.0 2,165,617 - BPHYT_RS09550 0.11 -2.1 2,166,077 - BPHYT_RS09550 0.41 -0.1 2,166,082 + BPHYT_RS09550 0.41 -0.4 2,166,182 + BPHYT_RS09550 0.48 -0.7 2,166,183 - BPHYT_RS09550 0.48 -0.4 2,166,183 - BPHYT_RS09550 0.48 +0.2 2,166,183 - BPHYT_RS09550 0.48 -0.5
Or see this region's nucleotide sequence