Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09230 and BPHYT_RS09235 are separated by 353 nucleotides BPHYT_RS09235 and BPHYT_RS09240 are separated by 38 nucleotides BPHYT_RS09240 and BPHYT_RS09245 overlap by 4 nucleotides BPHYT_RS09245 and BPHYT_RS09250 are separated by 168 nucleotides
BPHYT_RS09230: BPHYT_RS09230 - NADPH:quinone reductase, at 2,090,010 to 2,091,032
_RS09230
BPHYT_RS09235: BPHYT_RS09235 - hypothetical protein, at 2,091,386 to 2,091,943
_RS09235
BPHYT_RS09240: BPHYT_RS09240 - ABC transporter permease, at 2,091,982 to 2,092,788
_RS09240
BPHYT_RS09245: BPHYT_RS09245 - ABC transporter ATP-binding protein, at 2,092,785 to 2,093,513
_RS09245
BPHYT_RS09250: BPHYT_RS09250 - cell envelope biogenesis protein AsmA, at 2,093,682 to 2,096,180
_RS09250
Position (kb)
2091
2092
2093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2091.127 kb on + strand at 2091.142 kb on + strand at 2091.285 kb on - strand at 2091.342 kb on - strand at 2091.386 kb on - strand at 2091.386 kb on - strand at 2091.526 kb on + strand, within BPHYT_RS09235 at 2091.526 kb on + strand, within BPHYT_RS09235 at 2091.526 kb on + strand, within BPHYT_RS09235 at 2091.662 kb on + strand, within BPHYT_RS09235 at 2091.663 kb on - strand, within BPHYT_RS09235 at 2092.055 kb on + strand at 2092.378 kb on - strand, within BPHYT_RS09240 at 2092.413 kb on - strand, within BPHYT_RS09240 at 2092.413 kb on - strand, within BPHYT_RS09240 at 2092.598 kb on + strand, within BPHYT_RS09240 at 2092.598 kb on + strand, within BPHYT_RS09240 at 2092.599 kb on - strand, within BPHYT_RS09240 at 2092.654 kb on + strand, within BPHYT_RS09240 at 2092.654 kb on + strand, within BPHYT_RS09240 at 2092.655 kb on - strand, within BPHYT_RS09240 at 2092.655 kb on - strand, within BPHYT_RS09240 at 2093.257 kb on + strand, within BPHYT_RS09245 at 2093.258 kb on - strand, within BPHYT_RS09245 at 2093.367 kb on + strand, within BPHYT_RS09245 at 2093.496 kb on + strand at 2093.497 kb on - strand at 2093.497 kb on - strand at 2093.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 2,091,127 + +0.9 2,091,142 + -3.1 2,091,285 - +0.2 2,091,342 - +0.9 2,091,386 - +0.1 2,091,386 - +0.9 2,091,526 + BPHYT_RS09235 0.25 -0.0 2,091,526 + BPHYT_RS09235 0.25 +1.1 2,091,526 + BPHYT_RS09235 0.25 +1.0 2,091,662 + BPHYT_RS09235 0.49 +1.1 2,091,663 - BPHYT_RS09235 0.50 +0.9 2,092,055 + +0.5 2,092,378 - BPHYT_RS09240 0.49 +0.5 2,092,413 - BPHYT_RS09240 0.53 +0.7 2,092,413 - BPHYT_RS09240 0.53 -2.1 2,092,598 + BPHYT_RS09240 0.76 -0.7 2,092,598 + BPHYT_RS09240 0.76 +2.0 2,092,599 - BPHYT_RS09240 0.76 +1.8 2,092,654 + BPHYT_RS09240 0.83 -0.4 2,092,654 + BPHYT_RS09240 0.83 -1.1 2,092,655 - BPHYT_RS09240 0.83 +0.5 2,092,655 - BPHYT_RS09240 0.83 -1.4 2,093,257 + BPHYT_RS09245 0.65 +0.1 2,093,258 - BPHYT_RS09245 0.65 -0.1 2,093,367 + BPHYT_RS09245 0.80 -2.1 2,093,496 + -1.2 2,093,497 - +1.3 2,093,497 - -2.0 2,093,540 - -1.8
Or see this region's nucleotide sequence