Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08410 and BPHYT_RS08415 are separated by 30 nucleotides BPHYT_RS08415 and BPHYT_RS08420 are separated by 136 nucleotides BPHYT_RS08420 and BPHYT_RS08425 are separated by 129 nucleotides
BPHYT_RS08410: BPHYT_RS08410 - dehydrogenase, at 1,927,708 to 1,928,649
_RS08410
BPHYT_RS08415: BPHYT_RS08415 - hypothetical protein, at 1,928,680 to 1,929,063
_RS08415
BPHYT_RS08420: BPHYT_RS08420 - histidine kinase, at 1,929,200 to 1,929,637
_RS08420
BPHYT_RS08425: BPHYT_RS08425 - hypothetical protein, at 1,929,767 to 1,930,639
_RS08425
Position (kb) 1928
1929
1930 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1928.065 kb on - strand, within BPHYT_RS08410 at 1928.593 kb on + strand at 1928.628 kb on + strand at 1928.898 kb on + strand, within BPHYT_RS08415 at 1928.929 kb on + strand, within BPHYT_RS08415 at 1928.948 kb on - strand, within BPHYT_RS08415 at 1929.294 kb on - strand, within BPHYT_RS08420 at 1929.889 kb on + strand, within BPHYT_RS08425 at 1929.928 kb on - strand, within BPHYT_RS08425 at 1930.020 kb on + strand, within BPHYT_RS08425 at 1930.020 kb on + strand, within BPHYT_RS08425 at 1930.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 1,928,065 - BPHYT_RS08410 0.38 -1.4 1,928,593 + +0.2 1,928,628 + -0.1 1,928,898 + BPHYT_RS08415 0.57 -1.6 1,928,929 + BPHYT_RS08415 0.65 -1.5 1,928,948 - BPHYT_RS08415 0.70 -1.9 1,929,294 - BPHYT_RS08420 0.21 -1.1 1,929,889 + BPHYT_RS08425 0.14 -0.8 1,929,928 - BPHYT_RS08425 0.18 +0.1 1,930,020 + BPHYT_RS08425 0.29 -0.9 1,930,020 + BPHYT_RS08425 0.29 -2.9 1,930,021 - -0.6
Or see this region's nucleotide sequence