Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05820 and BPHYT_RS05825 are separated by 32 nucleotides BPHYT_RS05825 and BPHYT_RS05830 are separated by 165 nucleotides BPHYT_RS05830 and BPHYT_RS05835 are separated by 32 nucleotides BPHYT_RS05835 and BPHYT_RS05840 overlap by 13 nucleotides BPHYT_RS05840 and BPHYT_RS05845 overlap by 4 nucleotides
BPHYT_RS05820: BPHYT_RS05820 - hypothetical protein, at 1,325,632 to 1,327,530
_RS05820
BPHYT_RS05825: BPHYT_RS05825 - hypothetical protein, at 1,327,563 to 1,327,784
_RS05825
BPHYT_RS05830: BPHYT_RS05830 - hypothetical protein, at 1,327,950 to 1,328,408
_RS05830
BPHYT_RS05835: BPHYT_RS05835 - hypothetical protein, at 1,328,441 to 1,328,845
_RS05835
BPHYT_RS05840: BPHYT_RS05840 - hypothetical protein, at 1,328,833 to 1,329,240
_RS05840
BPHYT_RS05845: BPHYT_RS05845 - hypothetical protein, at 1,329,237 to 1,329,659
_RS05845
Position (kb) 1327
1328
1329 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 1326.951 kb on + strand, within BPHYT_RS05820 at 1326.954 kb on + strand, within BPHYT_RS05820 at 1326.955 kb on - strand, within BPHYT_RS05820 at 1327.013 kb on - strand, within BPHYT_RS05820 at 1327.020 kb on + strand, within BPHYT_RS05820 at 1327.020 kb on + strand, within BPHYT_RS05820 at 1327.021 kb on - strand, within BPHYT_RS05820 at 1327.096 kb on - strand, within BPHYT_RS05820 at 1327.147 kb on + strand, within BPHYT_RS05820 at 1327.147 kb on + strand, within BPHYT_RS05820 at 1327.183 kb on + strand, within BPHYT_RS05820 at 1327.227 kb on - strand, within BPHYT_RS05820 at 1327.314 kb on + strand, within BPHYT_RS05820 at 1327.315 kb on - strand, within BPHYT_RS05820 at 1327.368 kb on + strand at 1327.490 kb on - strand at 1327.519 kb on + strand at 1327.693 kb on + strand, within BPHYT_RS05825 at 1327.694 kb on - strand, within BPHYT_RS05825 at 1327.898 kb on + strand at 1328.009 kb on - strand, within BPHYT_RS05830 at 1328.223 kb on + strand, within BPHYT_RS05830 at 1328.245 kb on + strand, within BPHYT_RS05830 at 1328.375 kb on - strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.472 kb on - strand at 1328.478 kb on - strand at 1328.489 kb on + strand, within BPHYT_RS05835 at 1328.646 kb on - strand, within BPHYT_RS05835 at 1328.649 kb on + strand, within BPHYT_RS05835 at 1328.649 kb on + strand, within BPHYT_RS05835 at 1328.649 kb on + strand, within BPHYT_RS05835 at 1328.650 kb on - strand, within BPHYT_RS05835 at 1328.654 kb on + strand, within BPHYT_RS05835 at 1328.654 kb on + strand, within BPHYT_RS05835 at 1328.685 kb on - strand, within BPHYT_RS05835 at 1328.705 kb on + strand, within BPHYT_RS05835 at 1328.876 kb on + strand, within BPHYT_RS05840 at 1328.877 kb on - strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840 at 1328.893 kb on + strand, within BPHYT_RS05840
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 1,326,951 + BPHYT_RS05820 0.69 +0.7 1,326,954 + BPHYT_RS05820 0.70 -0.9 1,326,955 - BPHYT_RS05820 0.70 +0.3 1,327,013 - BPHYT_RS05820 0.73 +1.4 1,327,020 + BPHYT_RS05820 0.73 +0.1 1,327,020 + BPHYT_RS05820 0.73 +0.2 1,327,021 - BPHYT_RS05820 0.73 +0.2 1,327,096 - BPHYT_RS05820 0.77 +1.2 1,327,147 + BPHYT_RS05820 0.80 -0.2 1,327,147 + BPHYT_RS05820 0.80 +1.2 1,327,183 + BPHYT_RS05820 0.82 +0.6 1,327,227 - BPHYT_RS05820 0.84 -1.8 1,327,314 + BPHYT_RS05820 0.89 +0.8 1,327,315 - BPHYT_RS05820 0.89 +0.3 1,327,368 + -1.5 1,327,490 - -3.1 1,327,519 + +0.3 1,327,693 + BPHYT_RS05825 0.59 -1.5 1,327,694 - BPHYT_RS05825 0.59 -1.3 1,327,898 + +0.5 1,328,009 - BPHYT_RS05830 0.13 +0.3 1,328,223 + BPHYT_RS05830 0.59 -0.1 1,328,245 + BPHYT_RS05830 0.64 -1.4 1,328,375 - +1.1 1,328,471 + -0.4 1,328,471 + +1.4 1,328,471 + -0.9 1,328,471 + -1.4 1,328,472 - +1.6 1,328,478 - -0.6 1,328,489 + BPHYT_RS05835 0.12 +0.5 1,328,646 - BPHYT_RS05835 0.51 +0.1 1,328,649 + BPHYT_RS05835 0.51 -1.7 1,328,649 + BPHYT_RS05835 0.51 +4.8 1,328,649 + BPHYT_RS05835 0.51 -2.9 1,328,650 - BPHYT_RS05835 0.52 -0.7 1,328,654 + BPHYT_RS05835 0.53 -1.9 1,328,654 + BPHYT_RS05835 0.53 +0.3 1,328,685 - BPHYT_RS05835 0.60 +0.1 1,328,705 + BPHYT_RS05835 0.65 +0.6 1,328,876 + BPHYT_RS05840 0.11 -0.9 1,328,877 - BPHYT_RS05840 0.11 +2.7 1,328,893 + BPHYT_RS05840 0.15 -0.0 1,328,893 + BPHYT_RS05840 0.15 -1.4 1,328,893 + BPHYT_RS05840 0.15 +0.7
Or see this region's nucleotide sequence