Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05630 and BPHYT_RS05635 are separated by 205 nucleotides BPHYT_RS05635 and BPHYT_RS05640 overlap by 2 nucleotides BPHYT_RS05640 and BPHYT_RS05645 are separated by 157 nucleotides BPHYT_RS05645 and BPHYT_RS05650 are separated by 324 nucleotides
BPHYT_RS05630: BPHYT_RS05630 - glutathione S-transferase, at 1,288,989 to 1,289,696
_RS05630
BPHYT_RS05635: BPHYT_RS05635 - hypothetical protein, at 1,289,902 to 1,290,159
_RS05635
BPHYT_RS05640: BPHYT_RS05640 - hypothetical protein, at 1,290,158 to 1,291,231
_RS05640
BPHYT_RS05645: BPHYT_RS05645 - hypothetical protein, at 1,291,389 to 1,291,607
_RS05645
BPHYT_RS05650: BPHYT_RS05650 - hypothetical protein, at 1,291,932 to 1,292,174
_RS05650
Position (kb) 1290
1291
1292 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1289.274 kb on + strand, within BPHYT_RS05630 at 1289.274 kb on + strand, within BPHYT_RS05630 at 1289.326 kb on - strand, within BPHYT_RS05630 at 1289.419 kb on - strand, within BPHYT_RS05630 at 1289.446 kb on - strand, within BPHYT_RS05630 at 1289.477 kb on - strand, within BPHYT_RS05630 at 1289.477 kb on - strand, within BPHYT_RS05630 at 1289.562 kb on - strand, within BPHYT_RS05630 at 1289.676 kb on - strand at 1289.682 kb on - strand at 1289.682 kb on - strand at 1289.895 kb on + strand at 1289.955 kb on + strand, within BPHYT_RS05635 at 1289.973 kb on + strand, within BPHYT_RS05635 at 1289.973 kb on + strand, within BPHYT_RS05635 at 1289.973 kb on + strand, within BPHYT_RS05635 at 1289.973 kb on + strand, within BPHYT_RS05635 at 1290.107 kb on + strand, within BPHYT_RS05635 at 1290.117 kb on + strand, within BPHYT_RS05635 at 1290.117 kb on + strand, within BPHYT_RS05635 at 1290.231 kb on + strand at 1290.239 kb on + strand at 1290.239 kb on + strand at 1290.239 kb on + strand at 1290.393 kb on - strand, within BPHYT_RS05640 at 1290.403 kb on + strand, within BPHYT_RS05640 at 1290.455 kb on + strand, within BPHYT_RS05640 at 1290.466 kb on - strand, within BPHYT_RS05640 at 1290.474 kb on + strand, within BPHYT_RS05640 at 1290.475 kb on - strand, within BPHYT_RS05640 at 1290.475 kb on - strand, within BPHYT_RS05640 at 1290.546 kb on - strand, within BPHYT_RS05640 at 1290.565 kb on - strand, within BPHYT_RS05640 at 1290.584 kb on + strand, within BPHYT_RS05640 at 1290.638 kb on + strand, within BPHYT_RS05640 at 1290.639 kb on - strand, within BPHYT_RS05640 at 1290.642 kb on - strand, within BPHYT_RS05640 at 1290.682 kb on + strand, within BPHYT_RS05640 at 1290.691 kb on + strand, within BPHYT_RS05640 at 1291.069 kb on + strand, within BPHYT_RS05640 at 1291.070 kb on - strand, within BPHYT_RS05640 at 1291.070 kb on - strand, within BPHYT_RS05640 at 1291.070 kb on - strand, within BPHYT_RS05640 at 1291.222 kb on - strand at 1291.222 kb on - strand at 1291.222 kb on - strand at 1291.345 kb on + strand at 1291.345 kb on + strand at 1291.345 kb on + strand at 1291.345 kb on + strand at 1291.524 kb on - strand, within BPHYT_RS05645 at 1291.548 kb on + strand, within BPHYT_RS05645 at 1291.548 kb on + strand, within BPHYT_RS05645 at 1291.549 kb on - strand, within BPHYT_RS05645 at 1291.593 kb on + strand at 1291.593 kb on + strand at 1291.594 kb on - strand at 1291.594 kb on - strand at 1291.594 kb on - strand at 1291.623 kb on - strand at 1291.697 kb on + strand at 1291.715 kb on - strand at 1291.734 kb on + strand at 1291.948 kb on - strand at 1292.029 kb on - strand, within BPHYT_RS05650 at 1292.105 kb on + strand, within BPHYT_RS05650 at 1292.131 kb on - strand, within BPHYT_RS05650
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 1,289,274 + BPHYT_RS05630 0.40 -0.1 1,289,274 + BPHYT_RS05630 0.40 +0.7 1,289,326 - BPHYT_RS05630 0.48 +0.5 1,289,419 - BPHYT_RS05630 0.61 -2.6 1,289,446 - BPHYT_RS05630 0.65 -2.0 1,289,477 - BPHYT_RS05630 0.69 +0.1 1,289,477 - BPHYT_RS05630 0.69 -1.2 1,289,562 - BPHYT_RS05630 0.81 -0.3 1,289,676 - +0.4 1,289,682 - +1.0 1,289,682 - +1.0 1,289,895 + +0.4 1,289,955 + BPHYT_RS05635 0.21 -2.0 1,289,973 + BPHYT_RS05635 0.28 -0.3 1,289,973 + BPHYT_RS05635 0.28 -0.4 1,289,973 + BPHYT_RS05635 0.28 +0.5 1,289,973 + BPHYT_RS05635 0.28 -0.0 1,290,107 + BPHYT_RS05635 0.79 +0.1 1,290,117 + BPHYT_RS05635 0.83 -0.9 1,290,117 + BPHYT_RS05635 0.83 -0.4 1,290,231 + -1.0 1,290,239 + +1.2 1,290,239 + -2.3 1,290,239 + -0.3 1,290,393 - BPHYT_RS05640 0.22 -1.1 1,290,403 + BPHYT_RS05640 0.23 +0.1 1,290,455 + BPHYT_RS05640 0.28 -0.3 1,290,466 - BPHYT_RS05640 0.29 -0.4 1,290,474 + BPHYT_RS05640 0.29 +0.1 1,290,475 - BPHYT_RS05640 0.30 -0.3 1,290,475 - BPHYT_RS05640 0.30 +1.2 1,290,546 - BPHYT_RS05640 0.36 -1.0 1,290,565 - BPHYT_RS05640 0.38 +2.0 1,290,584 + BPHYT_RS05640 0.40 -0.2 1,290,638 + BPHYT_RS05640 0.45 -0.2 1,290,639 - BPHYT_RS05640 0.45 +0.6 1,290,642 - BPHYT_RS05640 0.45 -0.2 1,290,682 + BPHYT_RS05640 0.49 -0.6 1,290,691 + BPHYT_RS05640 0.50 +1.2 1,291,069 + BPHYT_RS05640 0.85 +0.4 1,291,070 - BPHYT_RS05640 0.85 -0.7 1,291,070 - BPHYT_RS05640 0.85 +0.2 1,291,070 - BPHYT_RS05640 0.85 +1.7 1,291,222 - -0.1 1,291,222 - +1.0 1,291,222 - +1.0 1,291,345 + -2.0 1,291,345 + +0.7 1,291,345 + +2.1 1,291,345 + -0.0 1,291,524 - BPHYT_RS05645 0.62 +0.2 1,291,548 + BPHYT_RS05645 0.73 +0.1 1,291,548 + BPHYT_RS05645 0.73 +0.4 1,291,549 - BPHYT_RS05645 0.73 -0.9 1,291,593 + +1.2 1,291,593 + -0.2 1,291,594 - -3.4 1,291,594 - -0.4 1,291,594 - -0.3 1,291,623 - -0.6 1,291,697 + +0.2 1,291,715 - -1.1 1,291,734 + -1.7 1,291,948 - +1.0 1,292,029 - BPHYT_RS05650 0.40 +0.7 1,292,105 + BPHYT_RS05650 0.71 -0.8 1,292,131 - BPHYT_RS05650 0.82 -0.8
Or see this region's nucleotide sequence