Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03315 and BPHYT_RS03320 are separated by 87 nucleotides BPHYT_RS03320 and BPHYT_RS03325 are separated by 25 nucleotides BPHYT_RS03325 and BPHYT_RS03330 are separated by 79 nucleotides
BPHYT_RS03315: BPHYT_RS03315 - polymerase, at 747,186 to 748,307
_RS03315
BPHYT_RS03320: BPHYT_RS03320 - lactoylglutathione lyase, at 748,395 to 748,781
_RS03320
BPHYT_RS03325: BPHYT_RS03325 - hypothetical protein, at 748,807 to 749,205
_RS03325
BPHYT_RS03330: BPHYT_RS03330 - hypothetical protein, at 749,285 to 750,187
_RS03330
Position (kb) 748
749 Strain fitness (log2 ratio) -2
-1
0
1
2 at 747.422 kb on + strand, within BPHYT_RS03315 at 747.422 kb on + strand, within BPHYT_RS03315 at 747.423 kb on - strand, within BPHYT_RS03315 at 747.423 kb on - strand, within BPHYT_RS03315 at 747.450 kb on + strand, within BPHYT_RS03315 at 747.451 kb on - strand, within BPHYT_RS03315 at 747.552 kb on + strand, within BPHYT_RS03315 at 747.678 kb on + strand, within BPHYT_RS03315 at 747.678 kb on + strand, within BPHYT_RS03315 at 747.690 kb on + strand, within BPHYT_RS03315 at 747.690 kb on + strand, within BPHYT_RS03315 at 747.777 kb on + strand, within BPHYT_RS03315 at 747.880 kb on - strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.913 kb on - strand, within BPHYT_RS03315 at 747.913 kb on - strand, within BPHYT_RS03315 at 747.913 kb on - strand, within BPHYT_RS03315 at 747.915 kb on - strand, within BPHYT_RS03315 at 747.993 kb on + strand, within BPHYT_RS03315 at 747.993 kb on + strand, within BPHYT_RS03315 at 748.131 kb on + strand, within BPHYT_RS03315 at 748.131 kb on + strand, within BPHYT_RS03315 at 748.150 kb on - strand, within BPHYT_RS03315 at 748.177 kb on - strand, within BPHYT_RS03315 at 748.290 kb on - strand at 748.290 kb on - strand at 748.373 kb on - strand at 748.452 kb on + strand, within BPHYT_RS03320 at 748.505 kb on + strand, within BPHYT_RS03320 at 748.591 kb on - strand, within BPHYT_RS03320 at 748.749 kb on + strand at 748.780 kb on - strand at 748.813 kb on - strand at 749.057 kb on - strand, within BPHYT_RS03325 at 749.089 kb on + strand, within BPHYT_RS03325 at 749.130 kb on + strand, within BPHYT_RS03325 at 749.265 kb on + strand at 749.565 kb on + strand, within BPHYT_RS03330
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 747,422 + BPHYT_RS03315 0.21 -1.0 747,422 + BPHYT_RS03315 0.21 -0.2 747,423 - BPHYT_RS03315 0.21 -0.5 747,423 - BPHYT_RS03315 0.21 +0.3 747,450 + BPHYT_RS03315 0.24 -1.8 747,451 - BPHYT_RS03315 0.24 +0.1 747,552 + BPHYT_RS03315 0.33 +2.2 747,678 + BPHYT_RS03315 0.44 -2.1 747,678 + BPHYT_RS03315 0.44 -0.1 747,690 + BPHYT_RS03315 0.45 +1.0 747,690 + BPHYT_RS03315 0.45 -0.1 747,777 + BPHYT_RS03315 0.53 +0.5 747,880 - BPHYT_RS03315 0.62 -1.3 747,912 + BPHYT_RS03315 0.65 -0.6 747,912 + BPHYT_RS03315 0.65 +0.0 747,912 + BPHYT_RS03315 0.65 -1.2 747,912 + BPHYT_RS03315 0.65 -0.1 747,912 + BPHYT_RS03315 0.65 +1.5 747,913 - BPHYT_RS03315 0.65 -0.5 747,913 - BPHYT_RS03315 0.65 +1.5 747,913 - BPHYT_RS03315 0.65 +0.4 747,915 - BPHYT_RS03315 0.65 -0.9 747,993 + BPHYT_RS03315 0.72 -1.1 747,993 + BPHYT_RS03315 0.72 -0.8 748,131 + BPHYT_RS03315 0.84 +1.0 748,131 + BPHYT_RS03315 0.84 -1.6 748,150 - BPHYT_RS03315 0.86 -2.3 748,177 - BPHYT_RS03315 0.88 -2.7 748,290 - -0.2 748,290 - -1.0 748,373 - -1.7 748,452 + BPHYT_RS03320 0.15 -0.1 748,505 + BPHYT_RS03320 0.28 +0.5 748,591 - BPHYT_RS03320 0.51 +0.7 748,749 + +1.2 748,780 - +0.5 748,813 - +0.6 749,057 - BPHYT_RS03325 0.63 +1.3 749,089 + BPHYT_RS03325 0.71 +0.8 749,130 + BPHYT_RS03325 0.81 +0.4 749,265 + -0.1 749,565 + BPHYT_RS03330 0.31 -1.1
Or see this region's nucleotide sequence