Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03250 and BPHYT_RS03255 are separated by 19 nucleotides BPHYT_RS03255 and BPHYT_RS03260 are separated by 160 nucleotides
BPHYT_RS03250: BPHYT_RS03250 - diguanylate cyclase, at 730,026 to 733,136
_RS03250
BPHYT_RS03255: BPHYT_RS03255 - glutathione S-transferase, at 733,156 to 733,770
_RS03255
BPHYT_RS03260: BPHYT_RS03260 - glycine cleavage system regulatory protein, at 733,931 to 734,860
_RS03260
Position (kb) 733
734 Strain fitness (log2 ratio) -2
-1
0
1 at 732.189 kb on + strand, within BPHYT_RS03250 at 732.190 kb on - strand, within BPHYT_RS03250 at 732.276 kb on + strand, within BPHYT_RS03250 at 732.409 kb on - strand, within BPHYT_RS03250 at 732.409 kb on - strand, within BPHYT_RS03250 at 732.715 kb on - strand, within BPHYT_RS03250 at 732.834 kb on + strand at 732.849 kb on + strand at 732.850 kb on - strand at 733.056 kb on + strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.111 kb on + strand at 733.112 kb on - strand at 733.566 kb on - strand, within BPHYT_RS03255 at 733.661 kb on + strand, within BPHYT_RS03255 at 733.662 kb on - strand, within BPHYT_RS03255 at 733.709 kb on - strand at 733.791 kb on + strand at 733.792 kb on - strand at 733.869 kb on + strand at 733.869 kb on + strand at 733.902 kb on + strand at 733.903 kb on - strand at 734.291 kb on + strand, within BPHYT_RS03260 at 734.291 kb on + strand, within BPHYT_RS03260 at 734.729 kb on + strand, within BPHYT_RS03260
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 732,189 + BPHYT_RS03250 0.70 +0.5 732,190 - BPHYT_RS03250 0.70 -0.4 732,276 + BPHYT_RS03250 0.72 +0.2 732,409 - BPHYT_RS03250 0.77 -1.1 732,409 - BPHYT_RS03250 0.77 -0.2 732,715 - BPHYT_RS03250 0.86 +0.6 732,834 + +0.6 732,849 + +0.5 732,850 - -1.3 733,056 + -0.1 733,057 - -0.9 733,057 - +0.5 733,057 - -2.2 733,111 + +1.7 733,112 - +0.2 733,566 - BPHYT_RS03255 0.67 -0.2 733,661 + BPHYT_RS03255 0.82 -1.4 733,662 - BPHYT_RS03255 0.82 -1.2 733,709 - -2.4 733,791 + +0.7 733,792 - +0.2 733,869 + -0.7 733,869 + -2.2 733,902 + -0.2 733,903 - -0.1 734,291 + BPHYT_RS03260 0.39 +0.0 734,291 + BPHYT_RS03260 0.39 -0.2 734,729 + BPHYT_RS03260 0.86 +0.0
Or see this region's nucleotide sequence