Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02735 and BPHYT_RS02740 are separated by 38 nucleotides BPHYT_RS02740 and BPHYT_RS02745 are separated by 109 nucleotides
BPHYT_RS02735: BPHYT_RS02735 - Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6) <small>(from data)</small>, at 612,575 to 613,582
_RS02735
BPHYT_RS02740: BPHYT_RS02740 - N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) <small>(from data)</small>, at 613,621 to 616,185
_RS02740
BPHYT_RS02745: BPHYT_RS02745 - N-acetylglucosamine-specific PTS system, IIBC components (nagE) <small>(from data)</small>, at 616,295 to 618,070
_RS02745
Position (kb) 613
614
615
616
617 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 613.013 kb on + strand, within BPHYT_RS02735 at 613.013 kb on + strand, within BPHYT_RS02735 at 613.013 kb on + strand, within BPHYT_RS02735 at 613.014 kb on - strand, within BPHYT_RS02735 at 613.014 kb on - strand, within BPHYT_RS02735 at 613.014 kb on - strand, within BPHYT_RS02735 at 613.014 kb on - strand, within BPHYT_RS02735 at 613.014 kb on - strand, within BPHYT_RS02735 at 613.014 kb on - strand, within BPHYT_RS02735 at 613.016 kb on - strand at 613.016 kb on - strand, within BPHYT_RS02735 at 613.112 kb on - strand, within BPHYT_RS02735 at 613.432 kb on + strand, within BPHYT_RS02735 at 613.497 kb on - strand at 613.642 kb on - strand at 613.642 kb on - strand at 613.642 kb on - strand at 613.779 kb on + strand at 613.854 kb on + strand at 613.854 kb on + strand at 614.912 kb on - strand, within BPHYT_RS02740 at 614.913 kb on + strand, within BPHYT_RS02740 at 614.914 kb on - strand, within BPHYT_RS02740 at 615.430 kb on + strand, within BPHYT_RS02740 at 615.431 kb on - strand, within BPHYT_RS02740 at 615.431 kb on - strand, within BPHYT_RS02740 at 615.431 kb on - strand, within BPHYT_RS02740 at 615.691 kb on - strand, within BPHYT_RS02740 at 616.201 kb on + strand at 616.228 kb on + strand at 616.228 kb on + strand at 616.228 kb on + strand at 616.232 kb on + strand at 616.232 kb on + strand at 616.643 kb on + strand, within BPHYT_RS02745 at 616.752 kb on + strand, within BPHYT_RS02745 at 616.753 kb on - strand, within BPHYT_RS02745 at 617.174 kb on + strand, within BPHYT_RS02745 at 617.174 kb on + strand, within BPHYT_RS02745
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 613,013 + BPHYT_RS02735 0.43 -1.1 613,013 + BPHYT_RS02735 0.43 +0.8 613,013 + BPHYT_RS02735 0.43 -0.3 613,014 - BPHYT_RS02735 0.44 -0.0 613,014 - BPHYT_RS02735 0.44 +0.0 613,014 - BPHYT_RS02735 0.44 -0.5 613,014 - BPHYT_RS02735 0.44 -0.3 613,014 - BPHYT_RS02735 0.44 -0.9 613,014 - BPHYT_RS02735 0.44 -0.7 613,016 - +3.0 613,016 - BPHYT_RS02735 0.44 -1.2 613,112 - BPHYT_RS02735 0.53 +1.6 613,432 + BPHYT_RS02735 0.85 -0.7 613,497 - -0.8 613,642 - -0.6 613,642 - -0.7 613,642 - -0.5 613,779 + -0.1 613,854 + +1.1 613,854 + -0.2 614,912 - BPHYT_RS02740 0.50 -0.2 614,913 + BPHYT_RS02740 0.50 +1.3 614,914 - BPHYT_RS02740 0.50 +0.6 615,430 + BPHYT_RS02740 0.71 -0.6 615,431 - BPHYT_RS02740 0.71 +1.1 615,431 - BPHYT_RS02740 0.71 +1.0 615,431 - BPHYT_RS02740 0.71 -0.6 615,691 - BPHYT_RS02740 0.81 +0.9 616,201 + +0.4 616,228 + -0.3 616,228 + +0.1 616,228 + -1.2 616,232 + -0.2 616,232 + +0.3 616,643 + BPHYT_RS02745 0.20 -3.2 616,752 + BPHYT_RS02745 0.26 +1.0 616,753 - BPHYT_RS02745 0.26 +0.7 617,174 + BPHYT_RS02745 0.49 +0.9 617,174 + BPHYT_RS02745 0.49 -0.4
Or see this region's nucleotide sequence