Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02095 and BPHYT_RS02100 are separated by 65 nucleotides BPHYT_RS02100 and BPHYT_RS02105 are separated by 111 nucleotides BPHYT_RS02105 and BPHYT_RS02110 are separated by 314 nucleotides
BPHYT_RS02095: BPHYT_RS02095 - alpha/beta hydrolase, at 483,161 to 484,051
_RS02095
BPHYT_RS02100: BPHYT_RS02100 - AsnC family transcriptional regulator, at 484,117 to 484,587
_RS02100
BPHYT_RS02105: BPHYT_RS02105 - multidrug DMT transporter permease, at 484,699 to 485,601
_RS02105
BPHYT_RS02110: BPHYT_RS02110 - beta-lactamase, at 485,916 to 486,836
_RS02110
Position (kb) 484
485
486 Strain fitness (log2 ratio) -2
-1
0
1 at 483.794 kb on + strand, within BPHYT_RS02095 at 483.980 kb on + strand at 483.980 kb on + strand at 483.981 kb on - strand at 483.981 kb on - strand at 483.981 kb on - strand at 484.050 kb on - strand at 484.050 kb on - strand at 484.236 kb on - strand, within BPHYT_RS02100 at 484.605 kb on - strand at 484.828 kb on - strand, within BPHYT_RS02105 at 484.865 kb on + strand, within BPHYT_RS02105 at 485.091 kb on - strand, within BPHYT_RS02105 at 485.201 kb on - strand, within BPHYT_RS02105 at 485.384 kb on - strand, within BPHYT_RS02105 at 485.799 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 483,794 + BPHYT_RS02095 0.71 -1.9 483,980 + +0.1 483,980 + +0.0 483,981 - +0.6 483,981 - -0.3 483,981 - -0.3 484,050 - -1.0 484,050 - +0.9 484,236 - BPHYT_RS02100 0.25 +0.5 484,605 - +0.9 484,828 - BPHYT_RS02105 0.14 -1.3 484,865 + BPHYT_RS02105 0.18 -1.0 485,091 - BPHYT_RS02105 0.43 -2.0 485,201 - BPHYT_RS02105 0.56 -1.0 485,384 - BPHYT_RS02105 0.76 -2.3 485,799 + -0.2
Or see this region's nucleotide sequence