Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00650 and BPHYT_RS00655 are separated by 25 nucleotides BPHYT_RS00655 and BPHYT_RS00660 are separated by 2 nucleotides
BPHYT_RS00650: BPHYT_RS00650 - acyl-CoA ligase, at 153,915 to 155,534
_RS00650
BPHYT_RS00655: BPHYT_RS00655 - 3-oxoacyl-ACP synthase, at 155,560 to 156,507
_RS00655
BPHYT_RS00660: BPHYT_RS00660 - 3-oxoacyl-ACP synthase, at 156,510 to 157,532
_RS00660
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 154.591 kb on + strand, within BPHYT_RS00650 at 154.591 kb on + strand, within BPHYT_RS00650 at 154.592 kb on - strand, within BPHYT_RS00650 at 154.667 kb on + strand, within BPHYT_RS00650 at 154.668 kb on - strand, within BPHYT_RS00650 at 154.678 kb on + strand, within BPHYT_RS00650 at 154.722 kb on - strand, within BPHYT_RS00650 at 155.007 kb on - strand, within BPHYT_RS00650 at 155.007 kb on - strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.009 kb on - strand, within BPHYT_RS00650 at 155.009 kb on - strand, within BPHYT_RS00650 at 155.009 kb on - strand, within BPHYT_RS00650 at 155.446 kb on + strand at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.858 kb on + strand, within BPHYT_RS00655 at 155.859 kb on - strand, within BPHYT_RS00655 at 155.859 kb on - strand, within BPHYT_RS00655 at 155.909 kb on + strand, within BPHYT_RS00655 at 155.924 kb on + strand, within BPHYT_RS00655 at 156.268 kb on - strand, within BPHYT_RS00655 at 156.512 kb on - strand at 156.523 kb on + strand at 156.524 kb on - strand at 156.524 kb on - strand at 156.524 kb on - strand at 156.615 kb on + strand, within BPHYT_RS00660 at 156.615 kb on + strand, within BPHYT_RS00660 at 156.615 kb on + strand, within BPHYT_RS00660 at 156.648 kb on + strand, within BPHYT_RS00660 at 156.649 kb on - strand, within BPHYT_RS00660 at 156.649 kb on - strand, within BPHYT_RS00660 at 156.649 kb on - strand, within BPHYT_RS00660 at 156.652 kb on + strand, within BPHYT_RS00660 at 156.734 kb on + strand, within BPHYT_RS00660 at 156.734 kb on + strand, within BPHYT_RS00660 at 156.735 kb on - strand, within BPHYT_RS00660 at 156.869 kb on + strand, within BPHYT_RS00660 at 157.152 kb on + strand, within BPHYT_RS00660 at 157.298 kb on - strand, within BPHYT_RS00660 at 157.384 kb on + strand, within BPHYT_RS00660 at 157.385 kb on - strand, within BPHYT_RS00660 at 157.437 kb on + strand at 157.474 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 154,591 + BPHYT_RS00650 0.42 -0.2 154,591 + BPHYT_RS00650 0.42 -2.0 154,592 - BPHYT_RS00650 0.42 -1.8 154,667 + BPHYT_RS00650 0.46 -0.4 154,668 - BPHYT_RS00650 0.46 +0.4 154,678 + BPHYT_RS00650 0.47 -1.0 154,722 - BPHYT_RS00650 0.50 -0.5 155,007 - BPHYT_RS00650 0.67 -0.2 155,007 - BPHYT_RS00650 0.67 +0.6 155,008 + BPHYT_RS00650 0.67 -0.5 155,008 + BPHYT_RS00650 0.67 -0.6 155,008 + BPHYT_RS00650 0.67 +0.6 155,008 + BPHYT_RS00650 0.67 +0.6 155,009 - BPHYT_RS00650 0.68 +0.2 155,009 - BPHYT_RS00650 0.68 -1.4 155,009 - BPHYT_RS00650 0.68 -1.4 155,446 + -0.7 155,711 + BPHYT_RS00655 0.16 +1.3 155,711 + BPHYT_RS00655 0.16 -1.6 155,711 + BPHYT_RS00655 0.16 -0.8 155,711 + BPHYT_RS00655 0.16 +0.7 155,711 + BPHYT_RS00655 0.16 -0.8 155,711 + BPHYT_RS00655 0.16 -0.1 155,711 + BPHYT_RS00655 0.16 -0.8 155,711 + BPHYT_RS00655 0.16 -0.4 155,858 + BPHYT_RS00655 0.31 -1.4 155,859 - BPHYT_RS00655 0.32 +0.4 155,859 - BPHYT_RS00655 0.32 -1.5 155,909 + BPHYT_RS00655 0.37 +0.5 155,924 + BPHYT_RS00655 0.38 +1.3 156,268 - BPHYT_RS00655 0.75 -1.5 156,512 - -1.2 156,523 + +0.5 156,524 - -0.2 156,524 - +0.5 156,524 - -0.9 156,615 + BPHYT_RS00660 0.10 -2.5 156,615 + BPHYT_RS00660 0.10 -1.1 156,615 + BPHYT_RS00660 0.10 +1.0 156,648 + BPHYT_RS00660 0.13 -0.3 156,649 - BPHYT_RS00660 0.14 +0.2 156,649 - BPHYT_RS00660 0.14 -0.6 156,649 - BPHYT_RS00660 0.14 -2.3 156,652 + BPHYT_RS00660 0.14 -2.9 156,734 + BPHYT_RS00660 0.22 +2.1 156,734 + BPHYT_RS00660 0.22 -0.0 156,735 - BPHYT_RS00660 0.22 +0.7 156,869 + BPHYT_RS00660 0.35 -1.0 157,152 + BPHYT_RS00660 0.63 -1.4 157,298 - BPHYT_RS00660 0.77 -0.4 157,384 + BPHYT_RS00660 0.85 -3.0 157,385 - BPHYT_RS00660 0.86 -0.8 157,437 + -0.2 157,474 + -0.2
Or see this region's nucleotide sequence