Experiment: 24hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31175 and BPHYT_RS31180 are separated by 6 nucleotides BPHYT_RS31180 and BPHYT_RS31185 are separated by 124 nucleotides BPHYT_RS31185 and BPHYT_RS31190 are separated by 141 nucleotides
BPHYT_RS31175: BPHYT_RS31175 - RNA polymerase sigma70 factor, at 2,552,511 to 2,553,785
_RS31175
BPHYT_RS31180: BPHYT_RS31180 - DGPFAETKE family protein, at 2,553,792 to 2,554,163
_RS31180
BPHYT_RS31185: BPHYT_RS31185 - LysR family transcriptional regulator, at 2,554,288 to 2,555,199
_RS31185
BPHYT_RS31190: BPHYT_RS31190 - ABC transporter permease, at 2,555,341 to 2,556,312
_RS31190
Position (kb)
2554
2555
2556 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2554.142 kb on - strand at 2554.157 kb on - strand at 2554.194 kb on - strand at 2554.198 kb on + strand at 2554.198 kb on + strand at 2554.199 kb on - strand at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.687 kb on + strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.408 kb on + strand at 2555.530 kb on + strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.996 kb on - strand, within BPHYT_RS31190
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 1 remove 2,554,142 - +0.1 2,554,157 - +1.7 2,554,194 - +3.0 2,554,198 + +0.4 2,554,198 + -0.0 2,554,199 - -2.1 2,554,390 + BPHYT_RS31185 0.11 +0.4 2,554,390 + BPHYT_RS31185 0.11 -0.6 2,554,687 + BPHYT_RS31185 0.44 +0.0 2,554,809 - BPHYT_RS31185 0.57 +0.5 2,554,809 - BPHYT_RS31185 0.57 +0.8 2,554,809 - BPHYT_RS31185 0.57 +0.6 2,555,220 - -0.7 2,555,220 - -1.0 2,555,220 - -0.1 2,555,220 - +0.5 2,555,408 + -0.4 2,555,530 + BPHYT_RS31190 0.19 -1.0 2,555,531 - BPHYT_RS31190 0.20 -0.5 2,555,531 - BPHYT_RS31190 0.20 +1.1 2,555,670 - BPHYT_RS31190 0.34 -0.6 2,555,670 - BPHYT_RS31190 0.34 +0.8 2,555,996 - BPHYT_RS31190 0.67 -0.9
Or see this region's nucleotide sequence