Experiment: 24hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28550 and BPHYT_RS28555 overlap by 4 nucleotides BPHYT_RS28555 and BPHYT_RS28560 overlap by 4 nucleotides BPHYT_RS28560 and BPHYT_RS28565 are separated by 9 nucleotides BPHYT_RS28565 and BPHYT_RS28570 are separated by 64 nucleotides
BPHYT_RS28550: BPHYT_RS28550 - ABC transporter, at 1,960,980 to 1,961,687
_RS28550
BPHYT_RS28555: BPHYT_RS28555 - branched-chain amino acid ABC transporter ATP-binding protein, at 1,961,684 to 1,962,394
_RS28555
BPHYT_RS28560: BPHYT_RS28560 - ABC transporter permease, at 1,962,391 to 1,963,461
_RS28560
BPHYT_RS28565: BPHYT_RS28565 - ABC transporter permease, at 1,963,471 to 1,964,340
_RS28565
BPHYT_RS28570: BPHYT_RS28570 - urea ABC transporter substrate-binding protein, at 1,964,405 to 1,965,640
_RS28570
Position (kb) 1962
1963
1964 Strain fitness (log2 ratio) -1
0
1
2 at 1962.704 kb on + strand, within BPHYT_RS28560 at 1962.995 kb on + strand, within BPHYT_RS28560 at 1963.107 kb on + strand, within BPHYT_RS28560 at 1963.232 kb on + strand, within BPHYT_RS28560 at 1963.472 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 1 remove 1,962,704 + BPHYT_RS28560 0.29 +0.0 1,962,995 + BPHYT_RS28560 0.56 +0.3 1,963,107 + BPHYT_RS28560 0.67 -0.4 1,963,232 + BPHYT_RS28560 0.79 +2.2 1,963,472 + -0.4
Or see this region's nucleotide sequence