Experiment: 24hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16515 and BPHYT_RS16520 are separated by 188 nucleotides BPHYT_RS16520 and BPHYT_RS16525 are separated by 16 nucleotides BPHYT_RS16525 and BPHYT_RS16530 are separated by 111 nucleotides
BPHYT_RS16515: BPHYT_RS16515 - 2-hydroxyacid dehydrogenase, at 3,744,174 to 3,744,863
_RS16515
BPHYT_RS16520: BPHYT_RS16520 - major facilitator superfamily protein, at 3,745,052 to 3,746,218
_RS16520
BPHYT_RS16525: BPHYT_RS16525 - extradiol dioxygenase, at 3,746,235 to 3,746,633
_RS16525
BPHYT_RS16530: BPHYT_RS16530 - LysR family transcriptional regulator, at 3,746,745 to 3,747,728
_RS16530
Position (kb)
3745
3746
3747 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3744.085 kb on + strand at 3744.085 kb on + strand at 3744.086 kb on - strand at 3744.086 kb on - strand at 3744.086 kb on - strand at 3744.118 kb on + strand at 3744.296 kb on + strand, within BPHYT_RS16515 at 3744.515 kb on - strand, within BPHYT_RS16515 at 3744.815 kb on + strand at 3744.815 kb on + strand at 3744.815 kb on + strand at 3744.816 kb on - strand at 3744.816 kb on - strand at 3744.905 kb on - strand at 3744.918 kb on + strand at 3744.918 kb on + strand at 3744.918 kb on + strand at 3744.919 kb on - strand at 3744.919 kb on - strand at 3744.919 kb on - strand at 3744.919 kb on - strand at 3744.919 kb on - strand at 3744.919 kb on - strand at 3744.949 kb on + strand at 3744.963 kb on + strand at 3744.964 kb on - strand at 3745.054 kb on - strand at 3745.054 kb on - strand at 3745.054 kb on - strand at 3745.091 kb on - strand at 3745.235 kb on - strand, within BPHYT_RS16520 at 3745.281 kb on - strand, within BPHYT_RS16520 at 3745.553 kb on + strand, within BPHYT_RS16520 at 3745.554 kb on - strand, within BPHYT_RS16520 at 3745.554 kb on - strand, within BPHYT_RS16520 at 3745.554 kb on - strand, within BPHYT_RS16520 at 3745.557 kb on + strand, within BPHYT_RS16520 at 3745.557 kb on + strand, within BPHYT_RS16520 at 3745.557 kb on + strand, within BPHYT_RS16520 at 3745.557 kb on + strand, within BPHYT_RS16520 at 3745.558 kb on - strand, within BPHYT_RS16520 at 3745.558 kb on - strand, within BPHYT_RS16520 at 3745.558 kb on - strand, within BPHYT_RS16520 at 3745.558 kb on - strand, within BPHYT_RS16520 at 3745.947 kb on + strand, within BPHYT_RS16520 at 3745.973 kb on - strand, within BPHYT_RS16520 at 3745.973 kb on - strand, within BPHYT_RS16520 at 3745.973 kb on - strand, within BPHYT_RS16520 at 3746.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 1 remove 3,744,085 + -0.2 3,744,085 + -0.9 3,744,086 - -0.2 3,744,086 - +0.2 3,744,086 - +0.7 3,744,118 + +1.3 3,744,296 + BPHYT_RS16515 0.18 -1.1 3,744,515 - BPHYT_RS16515 0.49 -2.4 3,744,815 + -0.0 3,744,815 + +0.2 3,744,815 + -0.0 3,744,816 - -1.1 3,744,816 - -0.2 3,744,905 - -0.2 3,744,918 + +2.2 3,744,918 + -0.6 3,744,918 + -1.0 3,744,919 - +0.2 3,744,919 - -0.7 3,744,919 - +0.1 3,744,919 - -0.4 3,744,919 - -1.7 3,744,919 - +0.3 3,744,949 + +0.4 3,744,963 + -1.1 3,744,964 - -0.3 3,745,054 - +0.5 3,745,054 - -0.0 3,745,054 - -1.7 3,745,091 - -1.5 3,745,235 - BPHYT_RS16520 0.16 -2.3 3,745,281 - BPHYT_RS16520 0.20 -1.9 3,745,553 + BPHYT_RS16520 0.43 +0.3 3,745,554 - BPHYT_RS16520 0.43 -0.1 3,745,554 - BPHYT_RS16520 0.43 -1.3 3,745,554 - BPHYT_RS16520 0.43 +0.4 3,745,557 + BPHYT_RS16520 0.43 -1.9 3,745,557 + BPHYT_RS16520 0.43 -1.2 3,745,557 + BPHYT_RS16520 0.43 -0.8 3,745,557 + BPHYT_RS16520 0.43 -0.3 3,745,558 - BPHYT_RS16520 0.43 -1.6 3,745,558 - BPHYT_RS16520 0.43 +0.1 3,745,558 - BPHYT_RS16520 0.43 +1.2 3,745,558 - BPHYT_RS16520 0.43 -1.1 3,745,947 + BPHYT_RS16520 0.77 -0.4 3,745,973 - BPHYT_RS16520 0.79 +0.3 3,745,973 - BPHYT_RS16520 0.79 -0.4 3,745,973 - BPHYT_RS16520 0.79 -0.0 3,746,237 - +0.0
Or see this region's nucleotide sequence