Experiment: 24hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15070 and BPHYT_RS15075 are separated by 121 nucleotides BPHYT_RS15075 and BPHYT_RS15080 are separated by 298 nucleotides BPHYT_RS15080 and BPHYT_RS15085 overlap by 1 nucleotides
BPHYT_RS15070: BPHYT_RS15070 - amidase, at 3,428,442 to 3,429,821
_RS15070
BPHYT_RS15075: BPHYT_RS15075 - dihydrokaempferol 4-reductase, at 3,429,943 to 3,430,452
_RS15075
BPHYT_RS15080: BPHYT_RS15080 - FAD-binding molybdopterin dehydrogenase, at 3,430,751 to 3,432,382
_RS15080
BPHYT_RS15085: BPHYT_RS15085 - aldehyde oxidase, at 3,432,382 to 3,434,784
_RS15085
Position (kb)
3430
3431
3432
3433 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3429.851 kb on + strand at 3429.852 kb on - strand at 3429.858 kb on + strand at 3429.870 kb on - strand at 3429.894 kb on + strand at 3430.034 kb on - strand, within BPHYT_RS15075 at 3430.220 kb on - strand, within BPHYT_RS15075 at 3430.220 kb on - strand, within BPHYT_RS15075 at 3430.667 kb on - strand at 3430.670 kb on + strand at 3430.671 kb on - strand at 3430.671 kb on - strand at 3430.730 kb on + strand at 3430.730 kb on + strand at 3430.730 kb on + strand at 3430.731 kb on - strand at 3430.731 kb on - strand at 3430.731 kb on - strand at 3430.735 kb on + strand at 3430.736 kb on + strand at 3430.736 kb on + strand at 3430.737 kb on - strand at 3430.737 kb on - strand at 3430.760 kb on + strand at 3430.760 kb on + strand at 3431.070 kb on + strand, within BPHYT_RS15080 at 3431.600 kb on + strand, within BPHYT_RS15080 at 3431.601 kb on - strand, within BPHYT_RS15080 at 3431.601 kb on - strand, within BPHYT_RS15080 at 3431.601 kb on - strand, within BPHYT_RS15080 at 3431.687 kb on + strand, within BPHYT_RS15080 at 3431.688 kb on - strand, within BPHYT_RS15080 at 3431.688 kb on - strand, within BPHYT_RS15080 at 3432.203 kb on + strand, within BPHYT_RS15080 at 3432.502 kb on + strand at 3432.503 kb on - strand at 3432.621 kb on - strand at 3432.715 kb on + strand, within BPHYT_RS15085 at 3432.716 kb on - strand, within BPHYT_RS15085 at 3432.971 kb on - strand, within BPHYT_RS15085 at 3432.971 kb on - strand, within BPHYT_RS15085 at 3432.991 kb on + strand, within BPHYT_RS15085 at 3433.074 kb on + strand, within BPHYT_RS15085 at 3433.075 kb on - strand, within BPHYT_RS15085 at 3433.075 kb on - strand, within BPHYT_RS15085 at 3433.100 kb on + strand, within BPHYT_RS15085 at 3433.100 kb on + strand, within BPHYT_RS15085 at 3433.100 kb on + strand, within BPHYT_RS15085 at 3433.100 kb on + strand, within BPHYT_RS15085 at 3433.101 kb on - strand, within BPHYT_RS15085 at 3433.101 kb on - strand, within BPHYT_RS15085 at 3433.270 kb on + strand, within BPHYT_RS15085 at 3433.271 kb on - strand, within BPHYT_RS15085 at 3433.276 kb on + strand, within BPHYT_RS15085 at 3433.305 kb on + strand, within BPHYT_RS15085
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 1 remove 3,429,851 + -0.9 3,429,852 - +1.0 3,429,858 + -0.0 3,429,870 - +0.2 3,429,894 + -0.1 3,430,034 - BPHYT_RS15075 0.18 +0.3 3,430,220 - BPHYT_RS15075 0.54 +2.3 3,430,220 - BPHYT_RS15075 0.54 +0.3 3,430,667 - -2.0 3,430,670 + -0.4 3,430,671 - -0.3 3,430,671 - -0.7 3,430,730 + -0.3 3,430,730 + -0.2 3,430,730 + +0.6 3,430,731 - -0.3 3,430,731 - -0.1 3,430,731 - -2.1 3,430,735 + -0.1 3,430,736 + +0.1 3,430,736 + -0.7 3,430,737 - -1.0 3,430,737 - -0.9 3,430,760 + +0.1 3,430,760 + -0.9 3,431,070 + BPHYT_RS15080 0.20 +0.3 3,431,600 + BPHYT_RS15080 0.52 -0.3 3,431,601 - BPHYT_RS15080 0.52 -0.7 3,431,601 - BPHYT_RS15080 0.52 -0.7 3,431,601 - BPHYT_RS15080 0.52 -0.9 3,431,687 + BPHYT_RS15080 0.57 -0.1 3,431,688 - BPHYT_RS15080 0.57 -0.8 3,431,688 - BPHYT_RS15080 0.57 -1.7 3,432,203 + BPHYT_RS15080 0.89 -0.9 3,432,502 + +1.4 3,432,503 - -1.1 3,432,621 - -0.4 3,432,715 + BPHYT_RS15085 0.14 +0.9 3,432,716 - BPHYT_RS15085 0.14 -0.2 3,432,971 - BPHYT_RS15085 0.25 -1.3 3,432,971 - BPHYT_RS15085 0.25 -1.2 3,432,991 + BPHYT_RS15085 0.25 +0.0 3,433,074 + BPHYT_RS15085 0.29 +0.5 3,433,075 - BPHYT_RS15085 0.29 -1.1 3,433,075 - BPHYT_RS15085 0.29 -0.7 3,433,100 + BPHYT_RS15085 0.30 +0.3 3,433,100 + BPHYT_RS15085 0.30 -0.1 3,433,100 + BPHYT_RS15085 0.30 -0.5 3,433,100 + BPHYT_RS15085 0.30 -1.5 3,433,101 - BPHYT_RS15085 0.30 +0.1 3,433,101 - BPHYT_RS15085 0.30 -0.6 3,433,270 + BPHYT_RS15085 0.37 +0.2 3,433,271 - BPHYT_RS15085 0.37 +1.5 3,433,276 + BPHYT_RS15085 0.37 -0.4 3,433,305 + BPHYT_RS15085 0.38 -0.3
Or see this region's nucleotide sequence