Experiment: 24hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11330 and BPHYT_RS11335 are separated by 277 nucleotides BPHYT_RS11335 and BPHYT_RS11340 are separated by 200 nucleotides BPHYT_RS11340 and BPHYT_RS11345 overlap by 4 nucleotides BPHYT_RS11345 and BPHYT_RS11350 are separated by 115 nucleotides BPHYT_RS11350 and BPHYT_RS11355 are separated by 127 nucleotides
BPHYT_RS11330: BPHYT_RS11330 - phospholipase, at 2,573,764 to 2,575,101
_RS11330
BPHYT_RS11335: BPHYT_RS11335 - hypothetical protein, at 2,575,379 to 2,575,630
_RS11335
BPHYT_RS11340: BPHYT_RS11340 - hypothetical protein, at 2,575,831 to 2,576,061
_RS11340
BPHYT_RS11345: BPHYT_RS11345 - AraC family transcriptional regulator, at 2,576,058 to 2,576,960
_RS11345
BPHYT_RS11350: BPHYT_RS11350 - cupin, at 2,577,076 to 2,577,465
_RS11350
BPHYT_RS11355: BPHYT_RS11355 - LysR family transcriptional regulator, at 2,577,593 to 2,578,654
_RS11355
Position (kb)
2576
2577 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2575.244 kb on + strand at 2575.244 kb on + strand at 2575.245 kb on - strand at 2575.472 kb on - strand, within BPHYT_RS11335 at 2575.540 kb on + strand, within BPHYT_RS11335 at 2575.561 kb on + strand, within BPHYT_RS11335 at 2575.641 kb on + strand at 2575.642 kb on - strand at 2575.812 kb on + strand at 2575.812 kb on + strand at 2575.812 kb on + strand at 2575.813 kb on - strand at 2575.816 kb on - strand at 2575.816 kb on - strand at 2575.816 kb on - strand at 2575.910 kb on - strand, within BPHYT_RS11340 at 2575.996 kb on + strand, within BPHYT_RS11340 at 2576.157 kb on + strand, within BPHYT_RS11345 at 2576.247 kb on - strand, within BPHYT_RS11345 at 2576.247 kb on - strand, within BPHYT_RS11345 at 2576.250 kb on + strand, within BPHYT_RS11345 at 2576.251 kb on - strand, within BPHYT_RS11345 at 2576.251 kb on - strand, within BPHYT_RS11345 at 2576.265 kb on - strand, within BPHYT_RS11345 at 2576.403 kb on + strand, within BPHYT_RS11345 at 2576.403 kb on + strand, within BPHYT_RS11345 at 2576.403 kb on + strand, within BPHYT_RS11345 at 2576.404 kb on - strand, within BPHYT_RS11345 at 2576.606 kb on + strand, within BPHYT_RS11345 at 2576.653 kb on - strand, within BPHYT_RS11345 at 2576.773 kb on - strand, within BPHYT_RS11345 at 2577.780 kb on + strand, within BPHYT_RS11355 at 2577.781 kb on - strand, within BPHYT_RS11355 at 2577.781 kb on - strand, within BPHYT_RS11355 at 2577.928 kb on - strand, within BPHYT_RS11355
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 1 remove 2,575,244 + -1.0 2,575,244 + -0.2 2,575,245 - +0.1 2,575,472 - BPHYT_RS11335 0.37 -0.7 2,575,540 + BPHYT_RS11335 0.64 -1.0 2,575,561 + BPHYT_RS11335 0.72 +0.3 2,575,641 + -0.2 2,575,642 - -0.1 2,575,812 + -0.9 2,575,812 + +0.6 2,575,812 + -1.5 2,575,813 - -0.8 2,575,816 - +3.4 2,575,816 - +3.0 2,575,816 - +0.1 2,575,910 - BPHYT_RS11340 0.34 -2.8 2,575,996 + BPHYT_RS11340 0.71 +0.6 2,576,157 + BPHYT_RS11345 0.11 -0.5 2,576,247 - BPHYT_RS11345 0.21 -0.8 2,576,247 - BPHYT_RS11345 0.21 +0.4 2,576,250 + BPHYT_RS11345 0.21 -0.1 2,576,251 - BPHYT_RS11345 0.21 -0.5 2,576,251 - BPHYT_RS11345 0.21 -0.4 2,576,265 - BPHYT_RS11345 0.23 +0.6 2,576,403 + BPHYT_RS11345 0.38 -0.8 2,576,403 + BPHYT_RS11345 0.38 -0.3 2,576,403 + BPHYT_RS11345 0.38 -1.6 2,576,404 - BPHYT_RS11345 0.38 +0.4 2,576,606 + BPHYT_RS11345 0.61 +0.1 2,576,653 - BPHYT_RS11345 0.66 +0.0 2,576,773 - BPHYT_RS11345 0.79 +1.1 2,577,780 + BPHYT_RS11355 0.18 -0.5 2,577,781 - BPHYT_RS11355 0.18 -0.5 2,577,781 - BPHYT_RS11355 0.18 -0.4 2,577,928 - BPHYT_RS11355 0.32 +0.2
Or see this region's nucleotide sequence