Experiment: 24hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10605 and BPHYT_RS10610 are separated by 275 nucleotides BPHYT_RS10610 and BPHYT_RS10615 are separated by 36 nucleotides BPHYT_RS10615 and BPHYT_RS10620 are separated by 56 nucleotides
BPHYT_RS10605: BPHYT_RS10605 - diguanylate cyclase, at 2,417,225 to 2,418,217
_RS10605
BPHYT_RS10610: BPHYT_RS10610 - LysR family transcriptional regulator, at 2,418,493 to 2,419,389
_RS10610
BPHYT_RS10615: BPHYT_RS10615 - phosphonoacetaldehyde hydrolase, at 2,419,426 to 2,420,229
_RS10615
BPHYT_RS10620: BPHYT_RS10620 - 2-aminoethylphosphonate:pyruvate aminotransferase, at 2,420,286 to 2,421,518
_RS10620
Position (kb)
2418
2419
2420 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2417.581 kb on - strand, within BPHYT_RS10605 at 2417.593 kb on - strand, within BPHYT_RS10605 at 2417.773 kb on - strand, within BPHYT_RS10605 at 2418.039 kb on + strand, within BPHYT_RS10605 at 2418.279 kb on - strand at 2418.344 kb on + strand at 2418.384 kb on - strand at 2418.836 kb on - strand, within BPHYT_RS10610 at 2418.936 kb on + strand, within BPHYT_RS10610 at 2418.936 kb on + strand, within BPHYT_RS10610 at 2418.937 kb on - strand, within BPHYT_RS10610 at 2419.395 kb on + strand at 2419.410 kb on + strand at 2419.411 kb on - strand at 2419.467 kb on - strand at 2419.505 kb on + strand at 2419.736 kb on + strand, within BPHYT_RS10615 at 2419.746 kb on - strand, within BPHYT_RS10615 at 2419.746 kb on - strand, within BPHYT_RS10615
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 1 remove 2,417,581 - BPHYT_RS10605 0.36 -0.8 2,417,593 - BPHYT_RS10605 0.37 -0.9 2,417,773 - BPHYT_RS10605 0.55 -0.1 2,418,039 + BPHYT_RS10605 0.82 +2.9 2,418,279 - -0.3 2,418,344 + +2.3 2,418,384 - -1.6 2,418,836 - BPHYT_RS10610 0.38 +0.0 2,418,936 + BPHYT_RS10610 0.49 +0.3 2,418,936 + BPHYT_RS10610 0.49 +0.2 2,418,937 - BPHYT_RS10610 0.49 -0.0 2,419,395 + -0.9 2,419,410 + -1.6 2,419,411 - +1.5 2,419,467 - +0.2 2,419,505 + +0.2 2,419,736 + BPHYT_RS10615 0.39 -0.7 2,419,746 - BPHYT_RS10615 0.40 -0.3 2,419,746 - BPHYT_RS10615 0.40 +0.1
Or see this region's nucleotide sequence