Experiment: 24hr PHYTO10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05285 and BPHYT_RS05290 are separated by 792 nucleotides BPHYT_RS05290 and BPHYT_RS05295 overlap by 26 nucleotides BPHYT_RS05295 and BPHYT_RS05300 are separated by 83 nucleotides BPHYT_RS05300 and BPHYT_RS05305 are separated by 74 nucleotides
BPHYT_RS05285: BPHYT_RS05285 - hypothetical protein, at 1,219,301 to 1,219,594
_RS05285
BPHYT_RS05290: BPHYT_RS05290 - hypothetical protein, at 1,220,387 to 1,220,890
_RS05290
BPHYT_RS05295: BPHYT_RS05295 - hypothetical protein, at 1,220,865 to 1,221,233
_RS05295
BPHYT_RS05300: BPHYT_RS05300 - polyketide cyclase, at 1,221,317 to 1,221,721
_RS05300
BPHYT_RS05305: BPHYT_RS05305 - LysR family transcriptional regulator, at 1,221,796 to 1,222,737
_RS05305
Position (kb) 1220
1221 Strain fitness (log2 ratio) -1
0
1 at 1219.613 kb on - strand at 1220.056 kb on - strand at 1220.331 kb on + strand at 1220.386 kb on + strand at 1220.818 kb on + strand, within BPHYT_RS05290 at 1220.819 kb on - strand, within BPHYT_RS05290 at 1221.035 kb on + strand, within BPHYT_RS05295 at 1221.359 kb on - strand, within BPHYT_RS05300 at 1221.359 kb on - strand, within BPHYT_RS05300 at 1221.387 kb on - strand, within BPHYT_RS05300 at 1221.472 kb on - strand, within BPHYT_RS05300 at 1221.670 kb on - strand, within BPHYT_RS05300 at 1221.691 kb on - strand at 1221.693 kb on - strand at 1221.766 kb on + strand at 1221.845 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 1 remove 1,219,613 - -0.6 1,220,056 - +0.4 1,220,331 + +0.2 1,220,386 + +1.0 1,220,818 + BPHYT_RS05290 0.86 -1.0 1,220,819 - BPHYT_RS05290 0.86 -0.5 1,221,035 + BPHYT_RS05295 0.46 +1.6 1,221,359 - BPHYT_RS05300 0.10 +0.1 1,221,359 - BPHYT_RS05300 0.10 -0.0 1,221,387 - BPHYT_RS05300 0.17 -0.3 1,221,472 - BPHYT_RS05300 0.38 +1.0 1,221,670 - BPHYT_RS05300 0.87 +1.1 1,221,691 - -1.3 1,221,693 - +0.9 1,221,766 + +0.7 1,221,845 - -0.8
Or see this region's nucleotide sequence