Experiment: 24hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28525 and BPHYT_RS28530 are separated by 28 nucleotides BPHYT_RS28530 and BPHYT_RS28535 are separated by 58 nucleotides BPHYT_RS28535 and BPHYT_RS28540 are separated by 1 nucleotides
BPHYT_RS28525: BPHYT_RS28525 - amidase, at 1,955,720 to 1,957,240
_RS28525
BPHYT_RS28530: BPHYT_RS28530 - 3-ketoacyl-ACP reductase, at 1,957,269 to 1,958,033
_RS28530
BPHYT_RS28535: BPHYT_RS28535 - response regulator receiver, at 1,958,092 to 1,958,742
_RS28535
BPHYT_RS28540: BPHYT_RS28540 - amino acid ABC substrate-binding protein, at 1,958,744 to 1,959,898
_RS28540
Position (kb) 1957
1958
1959 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1956.342 kb on + strand, within BPHYT_RS28525 at 1956.393 kb on + strand, within BPHYT_RS28525 at 1956.394 kb on - strand, within BPHYT_RS28525 at 1956.931 kb on - strand, within BPHYT_RS28525 at 1957.266 kb on - strand at 1957.837 kb on - strand, within BPHYT_RS28530 at 1958.100 kb on + strand at 1958.466 kb on - strand, within BPHYT_RS28535 at 1958.749 kb on - strand at 1958.800 kb on + strand at 1958.800 kb on + strand at 1958.803 kb on - strand at 1959.017 kb on - strand, within BPHYT_RS28540 at 1959.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10 remove 1,956,342 + BPHYT_RS28525 0.41 +0.0 1,956,393 + BPHYT_RS28525 0.44 -2.0 1,956,394 - BPHYT_RS28525 0.44 -0.2 1,956,931 - BPHYT_RS28525 0.80 -0.3 1,957,266 - +0.6 1,957,837 - BPHYT_RS28530 0.74 -0.6 1,958,100 + -0.4 1,958,466 - BPHYT_RS28535 0.57 +1.7 1,958,749 - +0.0 1,958,800 + +0.9 1,958,800 + +2.0 1,958,803 - +0.0 1,959,017 - BPHYT_RS28540 0.24 +0.7 1,959,017 - +1.4
Or see this region's nucleotide sequence