Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22500

Experiment: 24hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22490 and BPHYT_RS22495 are separated by 222 nucleotidesBPHYT_RS22495 and BPHYT_RS22500 are separated by 234 nucleotidesBPHYT_RS22500 and BPHYT_RS22505 are separated by 183 nucleotides BPHYT_RS22490: BPHYT_RS22490 - hypothetical protein, at 604,628 to 605,701 _RS22490 BPHYT_RS22495: BPHYT_RS22495 - hypothetical protein, at 605,924 to 606,232 _RS22495 BPHYT_RS22500: BPHYT_RS22500 - transcriptional regulator, at 606,467 to 607,471 _RS22500 BPHYT_RS22505: BPHYT_RS22505 - alpha/beta hydrolase, at 607,655 to 608,518 _RS22505 Position (kb) 606 607 608Strain fitness (log2 ratio) -1 0 1at 605.484 kb on + strand, within BPHYT_RS22490at 605.485 kb on - strand, within BPHYT_RS22490at 605.603 kb on - strandat 606.392 kb on - strandat 606.629 kb on - strand, within BPHYT_RS22500at 606.655 kb on - strand, within BPHYT_RS22500at 606.897 kb on + strand, within BPHYT_RS22500at 606.898 kb on - strand, within BPHYT_RS22500at 607.787 kb on - strand, within BPHYT_RS22505at 607.884 kb on - strand, within BPHYT_RS22505at 608.255 kb on - strand, within BPHYT_RS22505at 608.361 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10
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605,484 + BPHYT_RS22490 0.80 +0.4
605,485 - BPHYT_RS22490 0.80 +0.2
605,603 - -0.8
606,392 - +0.4
606,629 - BPHYT_RS22500 0.16 +1.4
606,655 - BPHYT_RS22500 0.19 +0.0
606,897 + BPHYT_RS22500 0.43 -1.1
606,898 - BPHYT_RS22500 0.43 -1.0
607,787 - BPHYT_RS22505 0.15 +0.2
607,884 - BPHYT_RS22505 0.27 +0.6
608,255 - BPHYT_RS22505 0.69 +0.6
608,361 - +0.8

Or see this region's nucleotide sequence