Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18640

Experiment: 24hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18635 and BPHYT_RS18640 are separated by 264 nucleotidesBPHYT_RS18640 and BPHYT_RS18645 overlap by 4 nucleotides BPHYT_RS18635: BPHYT_RS18635 - LamB/YcsF family protein, at 4,190,888 to 4,191,640 _RS18635 BPHYT_RS18640: BPHYT_RS18640 - membrane protein, at 4,191,905 to 4,192,654 _RS18640 BPHYT_RS18645: BPHYT_RS18645 - membrane protein, at 4,192,651 to 4,193,607 _RS18645 Position (kb) 4191 4192 4193Strain fitness (log2 ratio) -2 -1 0 1at 4191.122 kb on + strand, within BPHYT_RS18635at 4191.137 kb on + strand, within BPHYT_RS18635at 4191.138 kb on - strand, within BPHYT_RS18635at 4191.774 kb on - strandat 4191.774 kb on - strandat 4191.774 kb on - strandat 4191.774 kb on - strandat 4191.785 kb on + strandat 4191.925 kb on - strandat 4191.925 kb on - strandat 4191.925 kb on - strandat 4191.969 kb on + strandat 4191.969 kb on + strandat 4192.088 kb on - strand, within BPHYT_RS18640at 4192.214 kb on + strand, within BPHYT_RS18640at 4192.215 kb on - strand, within BPHYT_RS18640at 4192.215 kb on - strand, within BPHYT_RS18640at 4192.215 kb on - strandat 4192.669 kb on + strandat 4192.670 kb on - strandat 4192.670 kb on - strandat 4192.775 kb on - strand, within BPHYT_RS18645at 4192.829 kb on + strand, within BPHYT_RS18645at 4192.830 kb on - strand, within BPHYT_RS18645at 4193.255 kb on - strand, within BPHYT_RS18645at 4193.255 kb on - strand, within BPHYT_RS18645at 4193.294 kb on - strand, within BPHYT_RS18645at 4193.444 kb on - strand, within BPHYT_RS18645at 4193.574 kb on + strandat 4193.604 kb on - strandat 4193.605 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10
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4,191,122 + BPHYT_RS18635 0.31 +0.4
4,191,137 + BPHYT_RS18635 0.33 +0.5
4,191,138 - BPHYT_RS18635 0.33 -0.6
4,191,774 - +0.6
4,191,774 - -0.9
4,191,774 - -0.1
4,191,774 - -0.5
4,191,785 + -0.3
4,191,925 - +0.1
4,191,925 - +0.7
4,191,925 - -1.8
4,191,969 + +0.6
4,191,969 + +0.6
4,192,088 - BPHYT_RS18640 0.24 -0.3
4,192,214 + BPHYT_RS18640 0.41 +0.5
4,192,215 - BPHYT_RS18640 0.41 -0.7
4,192,215 - BPHYT_RS18640 0.41 +0.3
4,192,215 - +0.3
4,192,669 + +0.4
4,192,670 - +0.3
4,192,670 - -0.4
4,192,775 - BPHYT_RS18645 0.13 -0.6
4,192,829 + BPHYT_RS18645 0.19 +0.1
4,192,830 - BPHYT_RS18645 0.19 -0.2
4,193,255 - BPHYT_RS18645 0.63 -0.1
4,193,255 - BPHYT_RS18645 0.63 -0.6
4,193,294 - BPHYT_RS18645 0.67 -0.4
4,193,444 - BPHYT_RS18645 0.83 -1.8
4,193,574 + +0.2
4,193,604 - +0.2
4,193,605 + +0.2

Or see this region's nucleotide sequence