Experiment: 24hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15115 and BPHYT_RS15120 are separated by 60 nucleotides BPHYT_RS15120 and BPHYT_RS15125 are separated by 39 nucleotides BPHYT_RS15125 and BPHYT_RS15130 overlap by 4 nucleotides
BPHYT_RS15115: BPHYT_RS15115 - excinuclease ABC subunit A, at 3,440,843 to 3,441,286
_RS15115
BPHYT_RS15120: BPHYT_RS15120 - hypothetical protein, at 3,441,347 to 3,441,769
_RS15120
BPHYT_RS15125: BPHYT_RS15125 - 3-oxoacyl-ACP synthase, at 3,441,809 to 3,443,050
_RS15125
BPHYT_RS15130: BPHYT_RS15130 - 3-oxoacyl-ACP synthase, at 3,443,047 to 3,444,273
_RS15130
Position (kb)
3441
3442
3443
3444 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3440.844 kb on + strand at 3440.845 kb on - strand at 3440.845 kb on - strand at 3441.198 kb on + strand, within BPHYT_RS15115 at 3441.199 kb on - strand, within BPHYT_RS15115 at 3441.448 kb on + strand, within BPHYT_RS15120 at 3441.642 kb on + strand, within BPHYT_RS15120 at 3441.752 kb on + strand at 3441.794 kb on - strand at 3441.864 kb on + strand at 3441.999 kb on + strand, within BPHYT_RS15125 at 3442.165 kb on - strand, within BPHYT_RS15125 at 3442.165 kb on - strand, within BPHYT_RS15125 at 3442.288 kb on + strand, within BPHYT_RS15125 at 3442.288 kb on + strand, within BPHYT_RS15125 at 3442.288 kb on + strand, within BPHYT_RS15125 at 3442.381 kb on - strand, within BPHYT_RS15125 at 3442.576 kb on - strand, within BPHYT_RS15125 at 3442.650 kb on + strand, within BPHYT_RS15125 at 3442.705 kb on - strand, within BPHYT_RS15125 at 3442.760 kb on + strand, within BPHYT_RS15125 at 3442.988 kb on - strand at 3443.076 kb on + strand at 3443.076 kb on + strand at 3443.077 kb on - strand at 3443.077 kb on - strand at 3443.077 kb on - strand at 3443.114 kb on + strand at 3443.114 kb on + strand at 3443.151 kb on - strand at 3443.178 kb on + strand, within BPHYT_RS15130 at 3443.179 kb on - strand, within BPHYT_RS15130 at 3443.204 kb on + strand, within BPHYT_RS15130 at 3443.279 kb on + strand, within BPHYT_RS15130 at 3443.279 kb on + strand, within BPHYT_RS15130 at 3443.546 kb on + strand, within BPHYT_RS15130 at 3443.547 kb on - strand, within BPHYT_RS15130 at 3443.547 kb on - strand, within BPHYT_RS15130 at 3443.657 kb on + strand at 3443.751 kb on - strand, within BPHYT_RS15130 at 3443.870 kb on + strand, within BPHYT_RS15130 at 3443.871 kb on - strand, within BPHYT_RS15130 at 3443.871 kb on - strand, within BPHYT_RS15130 at 3443.961 kb on - strand, within BPHYT_RS15130 at 3444.047 kb on + strand, within BPHYT_RS15130 at 3444.047 kb on + strand, within BPHYT_RS15130 at 3444.048 kb on - strand, within BPHYT_RS15130
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10 remove 3,440,844 + -0.7 3,440,845 - -0.5 3,440,845 - -0.4 3,441,198 + BPHYT_RS15115 0.80 +0.1 3,441,199 - BPHYT_RS15115 0.80 -0.3 3,441,448 + BPHYT_RS15120 0.24 -0.5 3,441,642 + BPHYT_RS15120 0.70 -0.9 3,441,752 + -0.9 3,441,794 - +0.8 3,441,864 + +0.6 3,441,999 + BPHYT_RS15125 0.15 +0.1 3,442,165 - BPHYT_RS15125 0.29 +0.2 3,442,165 - BPHYT_RS15125 0.29 -0.3 3,442,288 + BPHYT_RS15125 0.39 -1.6 3,442,288 + BPHYT_RS15125 0.39 -0.2 3,442,288 + BPHYT_RS15125 0.39 +0.1 3,442,381 - BPHYT_RS15125 0.46 +0.7 3,442,576 - BPHYT_RS15125 0.62 +0.0 3,442,650 + BPHYT_RS15125 0.68 -1.3 3,442,705 - BPHYT_RS15125 0.72 -0.1 3,442,760 + BPHYT_RS15125 0.77 +0.9 3,442,988 - +1.8 3,443,076 + -0.8 3,443,076 + +1.4 3,443,077 - +0.2 3,443,077 - -0.8 3,443,077 - +0.6 3,443,114 + +0.7 3,443,114 + -0.2 3,443,151 - +0.9 3,443,178 + BPHYT_RS15130 0.11 +1.3 3,443,179 - BPHYT_RS15130 0.11 +0.4 3,443,204 + BPHYT_RS15130 0.13 -0.0 3,443,279 + BPHYT_RS15130 0.19 +0.4 3,443,279 + BPHYT_RS15130 0.19 +1.2 3,443,546 + BPHYT_RS15130 0.41 +0.8 3,443,547 - BPHYT_RS15130 0.41 +0.8 3,443,547 - BPHYT_RS15130 0.41 +1.3 3,443,657 + +3.2 3,443,751 - BPHYT_RS15130 0.57 +0.7 3,443,870 + BPHYT_RS15130 0.67 -0.2 3,443,871 - BPHYT_RS15130 0.67 -0.6 3,443,871 - BPHYT_RS15130 0.67 +0.5 3,443,961 - BPHYT_RS15130 0.74 -1.8 3,444,047 + BPHYT_RS15130 0.81 +0.0 3,444,047 + BPHYT_RS15130 0.81 -0.1 3,444,048 - BPHYT_RS15130 0.82 +0.5
Or see this region's nucleotide sequence