Experiment: 24hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00210 and BPHYT_RS00215 are separated by 10 nucleotides BPHYT_RS00215 and BPHYT_RS00220 are separated by 5 nucleotides BPHYT_RS00220 and BPHYT_RS00225 are separated by 169 nucleotides BPHYT_RS00225 and BPHYT_RS00230 overlap by 4 nucleotides
BPHYT_RS00210: BPHYT_RS00210 - allantoate amidohydrolase, at 55,474 to 56,766
_RS00210
BPHYT_RS00215: BPHYT_RS00215 - aminoglycoside phosphotransferase, at 56,777 to 57,727
_RS00215
BPHYT_RS00220: BPHYT_RS00220 - choline kinase, at 57,733 to 58,674
_RS00220
BPHYT_RS00225: BPHYT_RS00225 - hypothetical protein, at 58,844 to 59,089
_RS00225
BPHYT_RS00230: BPHYT_RS00230 - sodium:solute symporter, at 59,086 to 60,612
_RS00230
Position (kb) 57
58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.849 kb on - strand at 56.958 kb on - strand, within BPHYT_RS00215 at 57.044 kb on + strand, within BPHYT_RS00215 at 57.045 kb on - strand, within BPHYT_RS00215 at 57.543 kb on - strand, within BPHYT_RS00215 at 57.601 kb on - strand, within BPHYT_RS00215 at 57.601 kb on - strand, within BPHYT_RS00215 at 57.660 kb on + strand at 57.725 kb on + strand at 57.725 kb on + strand at 57.901 kb on + strand, within BPHYT_RS00220 at 58.014 kb on + strand, within BPHYT_RS00220 at 58.314 kb on + strand, within BPHYT_RS00220 at 58.315 kb on - strand, within BPHYT_RS00220 at 58.400 kb on - strand, within BPHYT_RS00220 at 58.469 kb on - strand, within BPHYT_RS00220 at 58.597 kb on + strand at 58.612 kb on + strand at 58.671 kb on - strand at 58.770 kb on + strand at 58.931 kb on + strand, within BPHYT_RS00225 at 59.037 kb on - strand, within BPHYT_RS00225 at 59.164 kb on + strand at 59.324 kb on - strand, within BPHYT_RS00230 at 59.362 kb on + strand, within BPHYT_RS00230 at 59.363 kb on - strand, within BPHYT_RS00230 at 59.363 kb on - strand, within BPHYT_RS00230 at 59.658 kb on + strand, within BPHYT_RS00230 at 59.659 kb on - strand, within BPHYT_RS00230 at 59.659 kb on - strand, within BPHYT_RS00230
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10 remove 56,849 - -0.6 56,958 - BPHYT_RS00215 0.19 -0.8 57,044 + BPHYT_RS00215 0.28 +0.0 57,045 - BPHYT_RS00215 0.28 -0.2 57,543 - BPHYT_RS00215 0.81 -0.9 57,601 - BPHYT_RS00215 0.87 +2.4 57,601 - BPHYT_RS00215 0.87 +1.5 57,660 + +0.2 57,725 + -0.7 57,725 + -1.2 57,901 + BPHYT_RS00220 0.18 -2.1 58,014 + BPHYT_RS00220 0.30 -1.3 58,314 + BPHYT_RS00220 0.62 +1.1 58,315 - BPHYT_RS00220 0.62 +0.3 58,400 - BPHYT_RS00220 0.71 -1.3 58,469 - BPHYT_RS00220 0.78 -0.6 58,597 + -1.0 58,612 + +0.9 58,671 - +0.8 58,770 + +0.1 58,931 + BPHYT_RS00225 0.35 +0.7 59,037 - BPHYT_RS00225 0.78 +1.3 59,164 + +0.0 59,324 - BPHYT_RS00230 0.16 +0.5 59,362 + BPHYT_RS00230 0.18 +0.4 59,363 - BPHYT_RS00230 0.18 +1.7 59,363 - BPHYT_RS00230 0.18 -0.4 59,658 + BPHYT_RS00230 0.37 -0.6 59,659 - BPHYT_RS00230 0.38 +1.2 59,659 - BPHYT_RS00230 0.38 +0.0
Or see this region's nucleotide sequence