Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30315

Experiment: 24hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30305 and BPHYT_RS30310 are separated by 29 nucleotidesBPHYT_RS30310 and BPHYT_RS30315 overlap by 4 nucleotidesBPHYT_RS30315 and BPHYT_RS30320 are separated by 127 nucleotides BPHYT_RS30305: BPHYT_RS30305 - ethyl tert-butyl ether degradation EthD, at 2,354,953 to 2,355,663 _RS30305 BPHYT_RS30310: BPHYT_RS30310 - LysR family transcriptional regulator, at 2,355,693 to 2,356,643 _RS30310 BPHYT_RS30315: BPHYT_RS30315 - LysR family transcriptional regulator, at 2,356,640 to 2,357,539 _RS30315 BPHYT_RS30320: BPHYT_RS30320 - MFS transporter, at 2,357,667 to 2,358,989 _RS30320 Position (kb) 2356 2357 2358Strain fitness (log2 ratio) -2 -1 0 1 2at 2355.645 kb on + strandat 2356.585 kb on - strandat 2356.946 kb on + strand, within BPHYT_RS30315at 2357.114 kb on - strand, within BPHYT_RS30315at 2357.114 kb on - strand, within BPHYT_RS30315at 2357.559 kb on + strandat 2357.751 kb on + strandat 2357.885 kb on - strand, within BPHYT_RS30320at 2358.049 kb on - strand, within BPHYT_RS30320at 2358.100 kb on - strand, within BPHYT_RS30320at 2358.163 kb on - strand, within BPHYT_RS30320at 2358.438 kb on - strand, within BPHYT_RS30320

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10
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2,355,645 + +2.4
2,356,585 - +0.8
2,356,946 + BPHYT_RS30315 0.34 -1.4
2,357,114 - BPHYT_RS30315 0.53 -2.3
2,357,114 - BPHYT_RS30315 0.53 -0.1
2,357,559 + +2.1
2,357,751 + -0.4
2,357,885 - BPHYT_RS30320 0.16 -0.4
2,358,049 - BPHYT_RS30320 0.29 +0.2
2,358,100 - BPHYT_RS30320 0.33 -0.9
2,358,163 - BPHYT_RS30320 0.37 -0.4
2,358,438 - BPHYT_RS30320 0.58 -0.2

Or see this region's nucleotide sequence