Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23275

Experiment: 24hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23265 and BPHYT_RS23270 are separated by 41 nucleotidesBPHYT_RS23270 and BPHYT_RS23275 are separated by 81 nucleotidesBPHYT_RS23275 and BPHYT_RS23280 are separated by 23 nucleotidesBPHYT_RS23280 and BPHYT_RS23285 are separated by 25 nucleotides BPHYT_RS23265: BPHYT_RS23265 - methylcrotonoyl-CoA carboxylase, at 783,565 to 785,172 _RS23265 BPHYT_RS23270: BPHYT_RS23270 - enoyl-CoA hydratase, at 785,214 to 785,999 _RS23270 BPHYT_RS23275: BPHYT_RS23275 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 786,081 to 788,159 _RS23275 BPHYT_RS23280: BPHYT_RS23280 - phosphate ABC transporter substrate-binding protein, at 788,183 to 788,974 _RS23280 BPHYT_RS23285: BPHYT_RS23285 - hypothetical protein, at 789,000 to 789,278 _RS23285 Position (kb) 786 787 788 789Strain fitness (log2 ratio) -2 -1 0 1at 785.658 kb on + strand, within BPHYT_RS23270at 786.684 kb on + strand, within BPHYT_RS23275at 786.747 kb on + strand, within BPHYT_RS23275at 786.882 kb on + strand, within BPHYT_RS23275at 787.095 kb on - strand, within BPHYT_RS23275at 787.391 kb on + strand, within BPHYT_RS23275at 788.200 kb on - strandat 788.408 kb on - strand, within BPHYT_RS23280at 788.615 kb on - strand, within BPHYT_RS23280at 788.635 kb on - strand, within BPHYT_RS23280at 788.944 kb on + strandat 788.945 kb on - strandat 788.946 kb on + strandat 788.947 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10
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785,658 + BPHYT_RS23270 0.56 +0.8
786,684 + BPHYT_RS23275 0.29 -1.1
786,747 + BPHYT_RS23275 0.32 -0.7
786,882 + BPHYT_RS23275 0.39 -0.5
787,095 - BPHYT_RS23275 0.49 -1.3
787,391 + BPHYT_RS23275 0.63 -2.4
788,200 - +0.4
788,408 - BPHYT_RS23280 0.28 -0.7
788,615 - BPHYT_RS23280 0.55 +0.5
788,635 - BPHYT_RS23280 0.57 -2.0
788,944 + -0.6
788,945 - -0.9
788,946 + -0.6
788,947 - +0.4

Or see this region's nucleotide sequence