Experiment: 24hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22950 and BPHYT_RS22955 are separated by 7 nucleotides BPHYT_RS22955 and BPHYT_RS22960 are separated by 63 nucleotides BPHYT_RS22960 and BPHYT_RS22965 are separated by 74 nucleotides
BPHYT_RS22950: BPHYT_RS22950 - von Willebrand factor A, at 706,467 to 707,822
_RS22950
BPHYT_RS22955: BPHYT_RS22955 - isoprenylcysteine carboxyl methyltransferase, at 707,830 to 708,543
_RS22955
BPHYT_RS22960: BPHYT_RS22960 - peptidase, at 708,607 to 710,145
_RS22960
BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634
_RS22965
Position (kb) 708
709
710
711 Strain fitness (log2 ratio) -2
-1
0
1 at 707.743 kb on - strand at 707.811 kb on - strand at 708.161 kb on - strand, within BPHYT_RS22955 at 708.325 kb on + strand, within BPHYT_RS22955 at 708.359 kb on - strand, within BPHYT_RS22955 at 708.548 kb on - strand at 709.994 kb on + strand at 709.995 kb on - strand at 710.273 kb on - strand at 710.341 kb on + strand at 710.483 kb on - strand, within BPHYT_RS22965 at 710.527 kb on + strand, within BPHYT_RS22965 at 710.767 kb on - strand, within BPHYT_RS22965 at 710.808 kb on + strand, within BPHYT_RS22965 at 710.808 kb on + strand, within BPHYT_RS22965 at 710.997 kb on - strand, within BPHYT_RS22965 at 710.998 kb on + strand, within BPHYT_RS22965
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10 remove 707,743 - -0.2 707,811 - +0.6 708,161 - BPHYT_RS22955 0.46 -0.9 708,325 + BPHYT_RS22955 0.69 -2.0 708,359 - BPHYT_RS22955 0.74 -1.0 708,548 - -0.1 709,994 + +0.7 709,995 - +0.2 710,273 - -0.8 710,341 + -1.1 710,483 - BPHYT_RS22965 0.11 +1.7 710,527 + BPHYT_RS22965 0.13 -0.1 710,767 - BPHYT_RS22965 0.23 +0.5 710,808 + BPHYT_RS22965 0.24 -1.5 710,808 + BPHYT_RS22965 0.24 +1.6 710,997 - BPHYT_RS22965 0.32 -0.3 710,998 + BPHYT_RS22965 0.32 +0.3
Or see this region's nucleotide sequence