Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22720

Experiment: 24hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22715 and BPHYT_RS22720 are separated by 178 nucleotidesBPHYT_RS22720 and BPHYT_RS22725 are separated by 195 nucleotides BPHYT_RS22715: BPHYT_RS22715 - L-2-keto-3-deoxyarabonate dehydratase (EC 4.2.1.43) (from data), at 651,362 to 652,294 _RS22715 BPHYT_RS22720: BPHYT_RS22720 - LysR family transcriptional regulator, at 652,473 to 653,456 _RS22720 BPHYT_RS22725: BPHYT_RS22725 - glycosyl transferase family 51, at 653,652 to 656,732 _RS22725 Position (kb) 652 653 654Strain fitness (log2 ratio) -2 -1 0 1 2at 651.598 kb on - strand, within BPHYT_RS22715at 651.598 kb on - strand, within BPHYT_RS22715at 651.916 kb on - strand, within BPHYT_RS22715at 651.916 kb on - strand, within BPHYT_RS22715at 652.024 kb on - strand, within BPHYT_RS22715at 652.408 kb on + strandat 652.411 kb on - strandat 652.411 kb on - strandat 652.760 kb on - strand, within BPHYT_RS22720at 652.974 kb on + strand, within BPHYT_RS22720at 652.975 kb on - strand, within BPHYT_RS22720at 652.981 kb on - strand, within BPHYT_RS22720at 653.091 kb on + strand, within BPHYT_RS22720at 653.593 kb on - strandat 653.812 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10
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651,598 - BPHYT_RS22715 0.25 +0.5
651,598 - BPHYT_RS22715 0.25 -0.2
651,916 - BPHYT_RS22715 0.59 +0.0
651,916 - BPHYT_RS22715 0.59 -0.6
652,024 - BPHYT_RS22715 0.71 +0.9
652,408 + -2.0
652,411 - -1.1
652,411 - +1.8
652,760 - BPHYT_RS22720 0.29 -0.5
652,974 + BPHYT_RS22720 0.51 -0.5
652,975 - BPHYT_RS22720 0.51 -1.5
652,981 - BPHYT_RS22720 0.52 -0.7
653,091 + BPHYT_RS22720 0.63 -0.3
653,593 - -0.7
653,812 - -0.6

Or see this region's nucleotide sequence