Experiment: 24hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21545 and BPHYT_RS21550 are separated by 168 nucleotides BPHYT_RS21550 and BPHYT_RS21555 are separated by 210 nucleotides BPHYT_RS21555 and BPHYT_RS21560 are separated by 258 nucleotides
BPHYT_RS21545: BPHYT_RS21545 - SAM-dependent methyltransferase, at 392,706 to 393,422
_RS21545
BPHYT_RS21550: BPHYT_RS21550 - hypothetical protein, at 393,591 to 394,109
_RS21550
BPHYT_RS21555: BPHYT_RS21555 - beta alanine--pyruvate aminotransferase, at 394,320 to 395,648
_RS21555
BPHYT_RS21560: BPHYT_RS21560 - GntR family transcriptional regulator, at 395,907 to 397,322
_RS21560
Position (kb) 394
395
396 Strain fitness (log2 ratio) -2
-1
0
1
2 at 393.333 kb on + strand, within BPHYT_RS21545 at 394.113 kb on - strand at 394.220 kb on + strand at 394.241 kb on + strand at 394.321 kb on + strand at 394.322 kb on - strand at 394.633 kb on + strand, within BPHYT_RS21555 at 394.684 kb on + strand, within BPHYT_RS21555 at 394.837 kb on + strand, within BPHYT_RS21555 at 395.536 kb on + strand at 395.539 kb on + strand at 395.540 kb on - strand at 395.651 kb on + strand at 395.652 kb on - strand at 395.724 kb on + strand at 395.724 kb on + strand at 395.724 kb on + strand at 395.835 kb on + strand at 395.835 kb on + strand at 395.860 kb on - strand at 395.860 kb on - strand at 395.860 kb on - strand at 395.917 kb on + strand at 395.918 kb on - strand at 396.084 kb on + strand, within BPHYT_RS21560 at 396.085 kb on - strand, within BPHYT_RS21560 at 396.085 kb on - strand, within BPHYT_RS21560 at 396.354 kb on + strand, within BPHYT_RS21560
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10 remove 393,333 + BPHYT_RS21545 0.87 +0.0 394,113 - -0.5 394,220 + -0.3 394,241 + -0.7 394,321 + -0.8 394,322 - +1.7 394,633 + BPHYT_RS21555 0.24 -0.8 394,684 + BPHYT_RS21555 0.27 -1.0 394,837 + BPHYT_RS21555 0.39 +0.1 395,536 + +0.0 395,539 + +0.4 395,540 - -0.1 395,651 + +1.4 395,652 - +1.8 395,724 + -0.3 395,724 + +0.0 395,724 + -1.0 395,835 + -2.6 395,835 + +1.1 395,860 - +0.4 395,860 - +2.2 395,860 - -0.7 395,917 + -1.0 395,918 - +0.7 396,084 + BPHYT_RS21560 0.12 +0.7 396,085 - BPHYT_RS21560 0.13 +0.0 396,085 - BPHYT_RS21560 0.13 +0.3 396,354 + BPHYT_RS21560 0.32 -0.7
Or see this region's nucleotide sequence