Experiment: 24hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17415 and BPHYT_RS17420 are separated by 128 nucleotides BPHYT_RS17420 and BPHYT_RS17425 are separated by 154 nucleotides
BPHYT_RS17415: BPHYT_RS17415 - uracil-DNA glycosylase, at 3,945,216 to 3,946,061
_RS17415
BPHYT_RS17420: BPHYT_RS17420 - FMN-dependent NADH-azoreductase, at 3,946,190 to 3,946,786
_RS17420
BPHYT_RS17425: BPHYT_RS17425 - peptidase M61, at 3,946,941 to 3,948,731
_RS17425
Position (kb)
3946
3947 Strain fitness (log2 ratio)
-2
-1
0
1 at 3946.059 kb on + strand at 3946.059 kb on + strand at 3946.191 kb on + strand at 3946.192 kb on - strand at 3946.269 kb on + strand, within BPHYT_RS17420 at 3946.380 kb on + strand, within BPHYT_RS17420 at 3946.381 kb on - strand, within BPHYT_RS17420 at 3946.381 kb on - strand, within BPHYT_RS17420 at 3946.381 kb on - strand, within BPHYT_RS17420 at 3946.436 kb on - strand, within BPHYT_RS17420 at 3946.464 kb on + strand, within BPHYT_RS17420 at 3946.464 kb on + strand, within BPHYT_RS17420 at 3946.464 kb on + strand, within BPHYT_RS17420 at 3946.465 kb on - strand, within BPHYT_RS17420 at 3946.497 kb on + strand, within BPHYT_RS17420 at 3946.891 kb on - strand at 3947.008 kb on + strand at 3947.008 kb on + strand at 3947.009 kb on - strand at 3947.009 kb on - strand at 3947.077 kb on - strand at 3947.447 kb on - strand, within BPHYT_RS17425 at 3947.508 kb on - strand, within BPHYT_RS17425 at 3947.566 kb on + strand, within BPHYT_RS17425 at 3947.566 kb on + strand, within BPHYT_RS17425 at 3947.569 kb on + strand, within BPHYT_RS17425 at 3947.569 kb on + strand, within BPHYT_RS17425 at 3947.569 kb on + strand, within BPHYT_RS17425 at 3947.570 kb on - strand, within BPHYT_RS17425 at 3947.666 kb on + strand, within BPHYT_RS17425 at 3947.758 kb on + strand, within BPHYT_RS17425 at 3947.759 kb on - strand, within BPHYT_RS17425 at 3947.759 kb on - strand, within BPHYT_RS17425 at 3947.761 kb on + strand, within BPHYT_RS17425 at 3947.761 kb on + strand, within BPHYT_RS17425 at 3947.762 kb on - strand, within BPHYT_RS17425
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10 remove 3,946,059 + +0.9 3,946,059 + -1.2 3,946,191 + -1.1 3,946,192 - +0.6 3,946,269 + BPHYT_RS17420 0.13 -0.6 3,946,380 + BPHYT_RS17420 0.32 +0.2 3,946,381 - BPHYT_RS17420 0.32 -0.7 3,946,381 - BPHYT_RS17420 0.32 +0.6 3,946,381 - BPHYT_RS17420 0.32 -0.4 3,946,436 - BPHYT_RS17420 0.41 +1.2 3,946,464 + BPHYT_RS17420 0.46 +0.1 3,946,464 + BPHYT_RS17420 0.46 -0.8 3,946,464 + BPHYT_RS17420 0.46 +0.4 3,946,465 - BPHYT_RS17420 0.46 -0.4 3,946,497 + BPHYT_RS17420 0.51 -0.6 3,946,891 - +0.2 3,947,008 + +0.0 3,947,008 + +0.6 3,947,009 - +0.1 3,947,009 - -0.1 3,947,077 - +0.3 3,947,447 - BPHYT_RS17425 0.28 -0.6 3,947,508 - BPHYT_RS17425 0.32 -0.4 3,947,566 + BPHYT_RS17425 0.35 -0.2 3,947,566 + BPHYT_RS17425 0.35 -0.1 3,947,569 + BPHYT_RS17425 0.35 +0.3 3,947,569 + BPHYT_RS17425 0.35 +1.2 3,947,569 + BPHYT_RS17425 0.35 -2.5 3,947,570 - BPHYT_RS17425 0.35 -1.2 3,947,666 + BPHYT_RS17425 0.40 -1.0 3,947,758 + BPHYT_RS17425 0.46 +1.2 3,947,759 - BPHYT_RS17425 0.46 -0.1 3,947,759 - BPHYT_RS17425 0.46 +0.0 3,947,761 + BPHYT_RS17425 0.46 +1.1 3,947,761 + BPHYT_RS17425 0.46 -0.6 3,947,762 - BPHYT_RS17425 0.46 -0.3
Or see this region's nucleotide sequence