Experiment: 24hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12180 and BPHYT_RS12185 are separated by 682 nucleotides BPHYT_RS12185 and BPHYT_RS12190 are separated by 70 nucleotides BPHYT_RS12190 and BPHYT_RS12195 overlap by 1 nucleotides
BPHYT_RS12180: BPHYT_RS12180 - multidrug DMT transporter permease, at 2,781,072 to 2,782,067
_RS12180
BPHYT_RS12185: BPHYT_RS12185 - succinyldiaminopimelate aminotransferase, at 2,782,750 to 2,783,967
_RS12185
BPHYT_RS12190: BPHYT_RS12190 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 2,784,038 to 2,784,865
_RS12190
BPHYT_RS12195: BPHYT_RS12195 - arsenate reductase, at 2,784,865 to 2,785,230
_RS12195
Position (kb)
2782
2783
2784 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2781.766 kb on + strand, within BPHYT_RS12180 at 2781.767 kb on - strand, within BPHYT_RS12180 at 2781.813 kb on + strand, within BPHYT_RS12180 at 2781.813 kb on + strand, within BPHYT_RS12180 at 2781.814 kb on - strand, within BPHYT_RS12180 at 2781.991 kb on + strand at 2782.374 kb on + strand at 2782.374 kb on + strand at 2782.374 kb on + strand at 2782.493 kb on + strand at 2782.494 kb on - strand at 2782.494 kb on - strand at 2782.591 kb on + strand at 2782.592 kb on - strand at 2782.619 kb on + strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.621 kb on + strand at 2782.622 kb on - strand at 2782.622 kb on - strand at 2782.842 kb on + strand at 2782.842 kb on + strand at 2782.994 kb on - strand, within BPHYT_RS12185 at 2782.994 kb on - strand, within BPHYT_RS12185 at 2783.121 kb on - strand, within BPHYT_RS12185 at 2783.121 kb on - strand, within BPHYT_RS12185 at 2783.121 kb on - strand, within BPHYT_RS12185 at 2783.140 kb on + strand, within BPHYT_RS12185 at 2783.150 kb on - strand, within BPHYT_RS12185 at 2783.185 kb on + strand, within BPHYT_RS12185 at 2783.185 kb on + strand, within BPHYT_RS12185 at 2783.196 kb on + strand, within BPHYT_RS12185 at 2783.196 kb on + strand, within BPHYT_RS12185 at 2783.272 kb on + strand, within BPHYT_RS12185 at 2783.272 kb on + strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.565 kb on - strand, within BPHYT_RS12185 at 2783.565 kb on - strand, within BPHYT_RS12185 at 2783.565 kb on - strand, within BPHYT_RS12185 at 2783.575 kb on + strand, within BPHYT_RS12185 at 2783.576 kb on - strand, within BPHYT_RS12185 at 2783.576 kb on - strand, within BPHYT_RS12185 at 2783.740 kb on + strand, within BPHYT_RS12185 at 2783.740 kb on + strand, within BPHYT_RS12185 at 2783.811 kb on + strand, within BPHYT_RS12185 at 2783.811 kb on + strand, within BPHYT_RS12185 at 2784.037 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10 remove 2,781,766 + BPHYT_RS12180 0.70 +0.4 2,781,767 - BPHYT_RS12180 0.70 +0.5 2,781,813 + BPHYT_RS12180 0.74 -0.0 2,781,813 + BPHYT_RS12180 0.74 -0.2 2,781,814 - BPHYT_RS12180 0.74 +1.2 2,781,991 + -0.3 2,782,374 + -0.4 2,782,374 + -2.0 2,782,374 + -0.5 2,782,493 + +0.6 2,782,494 - -0.3 2,782,494 - -0.2 2,782,591 + +0.0 2,782,592 - -0.2 2,782,619 + -1.5 2,782,620 - -0.1 2,782,620 - -1.1 2,782,620 - +0.4 2,782,620 - -1.1 2,782,620 - +0.5 2,782,621 + +1.2 2,782,622 - -0.7 2,782,622 - -2.5 2,782,842 + -0.5 2,782,842 + -0.0 2,782,994 - BPHYT_RS12185 0.20 +0.8 2,782,994 - BPHYT_RS12185 0.20 +0.1 2,783,121 - BPHYT_RS12185 0.30 +1.0 2,783,121 - BPHYT_RS12185 0.30 +1.0 2,783,121 - BPHYT_RS12185 0.30 +0.1 2,783,140 + BPHYT_RS12185 0.32 +1.2 2,783,150 - BPHYT_RS12185 0.33 -2.0 2,783,185 + BPHYT_RS12185 0.36 +1.3 2,783,185 + BPHYT_RS12185 0.36 +3.1 2,783,196 + BPHYT_RS12185 0.37 -2.1 2,783,196 + BPHYT_RS12185 0.37 -1.3 2,783,272 + BPHYT_RS12185 0.43 +1.7 2,783,272 + BPHYT_RS12185 0.43 +0.6 2,783,273 - BPHYT_RS12185 0.43 -0.9 2,783,273 - BPHYT_RS12185 0.43 +0.5 2,783,273 - BPHYT_RS12185 0.43 +0.6 2,783,273 - BPHYT_RS12185 0.43 +0.9 2,783,273 - BPHYT_RS12185 0.43 +1.7 2,783,273 - BPHYT_RS12185 0.43 +0.7 2,783,565 - BPHYT_RS12185 0.67 -1.2 2,783,565 - BPHYT_RS12185 0.67 +1.0 2,783,565 - BPHYT_RS12185 0.67 +0.8 2,783,575 + BPHYT_RS12185 0.68 -0.9 2,783,576 - BPHYT_RS12185 0.68 -1.5 2,783,576 - BPHYT_RS12185 0.68 +1.0 2,783,740 + BPHYT_RS12185 0.81 -0.6 2,783,740 + BPHYT_RS12185 0.81 -1.3 2,783,811 + BPHYT_RS12185 0.87 -0.1 2,783,811 + BPHYT_RS12185 0.87 +1.2 2,784,037 + -0.3
Or see this region's nucleotide sequence