Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12185

Experiment: 24hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12180 and BPHYT_RS12185 are separated by 682 nucleotidesBPHYT_RS12185 and BPHYT_RS12190 are separated by 70 nucleotidesBPHYT_RS12190 and BPHYT_RS12195 overlap by 1 nucleotides BPHYT_RS12180: BPHYT_RS12180 - multidrug DMT transporter permease, at 2,781,072 to 2,782,067 _RS12180 BPHYT_RS12185: BPHYT_RS12185 - succinyldiaminopimelate aminotransferase, at 2,782,750 to 2,783,967 _RS12185 BPHYT_RS12190: BPHYT_RS12190 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 2,784,038 to 2,784,865 _RS12190 BPHYT_RS12195: BPHYT_RS12195 - arsenate reductase, at 2,784,865 to 2,785,230 _RS12195 Position (kb) 2782 2783 2784Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2781.766 kb on + strand, within BPHYT_RS12180at 2781.767 kb on - strand, within BPHYT_RS12180at 2781.813 kb on + strand, within BPHYT_RS12180at 2781.813 kb on + strand, within BPHYT_RS12180at 2781.814 kb on - strand, within BPHYT_RS12180at 2781.991 kb on + strandat 2782.374 kb on + strandat 2782.374 kb on + strandat 2782.374 kb on + strandat 2782.493 kb on + strandat 2782.494 kb on - strandat 2782.494 kb on - strandat 2782.591 kb on + strandat 2782.592 kb on - strandat 2782.619 kb on + strandat 2782.620 kb on - strandat 2782.620 kb on - strandat 2782.620 kb on - strandat 2782.620 kb on - strandat 2782.620 kb on - strandat 2782.621 kb on + strandat 2782.622 kb on - strandat 2782.622 kb on - strandat 2782.842 kb on + strandat 2782.842 kb on + strandat 2782.994 kb on - strand, within BPHYT_RS12185at 2782.994 kb on - strand, within BPHYT_RS12185at 2783.121 kb on - strand, within BPHYT_RS12185at 2783.121 kb on - strand, within BPHYT_RS12185at 2783.121 kb on - strand, within BPHYT_RS12185at 2783.140 kb on + strand, within BPHYT_RS12185at 2783.150 kb on - strand, within BPHYT_RS12185at 2783.185 kb on + strand, within BPHYT_RS12185at 2783.185 kb on + strand, within BPHYT_RS12185at 2783.196 kb on + strand, within BPHYT_RS12185at 2783.196 kb on + strand, within BPHYT_RS12185at 2783.272 kb on + strand, within BPHYT_RS12185at 2783.272 kb on + strand, within BPHYT_RS12185at 2783.273 kb on - strand, within BPHYT_RS12185at 2783.273 kb on - strand, within BPHYT_RS12185at 2783.273 kb on - strand, within BPHYT_RS12185at 2783.273 kb on - strand, within BPHYT_RS12185at 2783.273 kb on - strand, within BPHYT_RS12185at 2783.273 kb on - strand, within BPHYT_RS12185at 2783.565 kb on - strand, within BPHYT_RS12185at 2783.565 kb on - strand, within BPHYT_RS12185at 2783.565 kb on - strand, within BPHYT_RS12185at 2783.575 kb on + strand, within BPHYT_RS12185at 2783.576 kb on - strand, within BPHYT_RS12185at 2783.576 kb on - strand, within BPHYT_RS12185at 2783.740 kb on + strand, within BPHYT_RS12185at 2783.740 kb on + strand, within BPHYT_RS12185at 2783.811 kb on + strand, within BPHYT_RS12185at 2783.811 kb on + strand, within BPHYT_RS12185at 2784.037 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10
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2,781,766 + BPHYT_RS12180 0.70 +0.4
2,781,767 - BPHYT_RS12180 0.70 +0.5
2,781,813 + BPHYT_RS12180 0.74 -0.0
2,781,813 + BPHYT_RS12180 0.74 -0.2
2,781,814 - BPHYT_RS12180 0.74 +1.2
2,781,991 + -0.3
2,782,374 + -0.4
2,782,374 + -2.0
2,782,374 + -0.5
2,782,493 + +0.6
2,782,494 - -0.3
2,782,494 - -0.2
2,782,591 + +0.0
2,782,592 - -0.2
2,782,619 + -1.5
2,782,620 - -0.1
2,782,620 - -1.1
2,782,620 - +0.4
2,782,620 - -1.1
2,782,620 - +0.5
2,782,621 + +1.2
2,782,622 - -0.7
2,782,622 - -2.5
2,782,842 + -0.5
2,782,842 + -0.0
2,782,994 - BPHYT_RS12185 0.20 +0.8
2,782,994 - BPHYT_RS12185 0.20 +0.1
2,783,121 - BPHYT_RS12185 0.30 +1.0
2,783,121 - BPHYT_RS12185 0.30 +1.0
2,783,121 - BPHYT_RS12185 0.30 +0.1
2,783,140 + BPHYT_RS12185 0.32 +1.2
2,783,150 - BPHYT_RS12185 0.33 -2.0
2,783,185 + BPHYT_RS12185 0.36 +1.3
2,783,185 + BPHYT_RS12185 0.36 +3.1
2,783,196 + BPHYT_RS12185 0.37 -2.1
2,783,196 + BPHYT_RS12185 0.37 -1.3
2,783,272 + BPHYT_RS12185 0.43 +1.7
2,783,272 + BPHYT_RS12185 0.43 +0.6
2,783,273 - BPHYT_RS12185 0.43 -0.9
2,783,273 - BPHYT_RS12185 0.43 +0.5
2,783,273 - BPHYT_RS12185 0.43 +0.6
2,783,273 - BPHYT_RS12185 0.43 +0.9
2,783,273 - BPHYT_RS12185 0.43 +1.7
2,783,273 - BPHYT_RS12185 0.43 +0.7
2,783,565 - BPHYT_RS12185 0.67 -1.2
2,783,565 - BPHYT_RS12185 0.67 +1.0
2,783,565 - BPHYT_RS12185 0.67 +0.8
2,783,575 + BPHYT_RS12185 0.68 -0.9
2,783,576 - BPHYT_RS12185 0.68 -1.5
2,783,576 - BPHYT_RS12185 0.68 +1.0
2,783,740 + BPHYT_RS12185 0.81 -0.6
2,783,740 + BPHYT_RS12185 0.81 -1.3
2,783,811 + BPHYT_RS12185 0.87 -0.1
2,783,811 + BPHYT_RS12185 0.87 +1.2
2,784,037 + -0.3

Or see this region's nucleotide sequence