Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS03235

Experiment: 24hr PHYTO10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS03230 and BPHYT_RS03235 are separated by 118 nucleotidesBPHYT_RS03235 and BPHYT_RS03240 are separated by 140 nucleotides BPHYT_RS03230: BPHYT_RS03230 - acetyl-CoA acetyltransferase, at 726,094 to 727,293 _RS03230 BPHYT_RS03235: BPHYT_RS03235 - enoyl-CoA hydratase, at 727,412 to 728,182 _RS03235 BPHYT_RS03240: BPHYT_RS03240 - formate dehydrogenase accessory protein FdhD, at 728,323 to 729,162 _RS03240 Position (kb) 727 728 729Strain fitness (log2 ratio) -1 0 1at 726.492 kb on + strand, within BPHYT_RS03230at 726.492 kb on + strand, within BPHYT_RS03230at 726.493 kb on - strand, within BPHYT_RS03230at 726.513 kb on + strand, within BPHYT_RS03230at 726.548 kb on - strand, within BPHYT_RS03230at 726.849 kb on + strand, within BPHYT_RS03230at 726.850 kb on - strand, within BPHYT_RS03230at 726.850 kb on - strand, within BPHYT_RS03230at 726.850 kb on - strand, within BPHYT_RS03230at 727.228 kb on + strandat 727.228 kb on + strandat 727.229 kb on - strandat 727.261 kb on - strandat 727.423 kb on + strandat 727.493 kb on - strand, within BPHYT_RS03235at 727.505 kb on + strand, within BPHYT_RS03235at 727.505 kb on + strand, within BPHYT_RS03235at 728.205 kb on + strandat 728.346 kb on + strandat 728.347 kb on - strandat 728.360 kb on + strandat 728.361 kb on - strandat 728.493 kb on - strand, within BPHYT_RS03240at 728.493 kb on - strand, within BPHYT_RS03240at 729.165 kb on + strandat 729.166 kb on - strandat 729.166 kb on - strandat 729.166 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO10 MOI 10
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726,492 + BPHYT_RS03230 0.33 -0.1
726,492 + BPHYT_RS03230 0.33 -0.6
726,493 - BPHYT_RS03230 0.33 +0.4
726,513 + BPHYT_RS03230 0.35 +0.0
726,548 - BPHYT_RS03230 0.38 +1.2
726,849 + BPHYT_RS03230 0.63 +0.2
726,850 - BPHYT_RS03230 0.63 +1.4
726,850 - BPHYT_RS03230 0.63 -1.1
726,850 - BPHYT_RS03230 0.63 +0.4
727,228 + +0.3
727,228 + -1.4
727,229 - -0.5
727,261 - -0.9
727,423 + +0.3
727,493 - BPHYT_RS03235 0.11 -0.4
727,505 + BPHYT_RS03235 0.12 -0.8
727,505 + BPHYT_RS03235 0.12 -0.2
728,205 + +0.7
728,346 + +0.5
728,347 - -1.1
728,360 + -1.1
728,361 - +0.5
728,493 - BPHYT_RS03240 0.20 -1.3
728,493 - BPHYT_RS03240 0.20 -0.6
729,165 + +0.5
729,166 - -1.6
729,166 - -1.3
729,166 - -0.4

Or see this region's nucleotide sequence