Experiment: 24hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35705 and BPHYT_RS35710 are separated by 65 nucleotides BPHYT_RS35710 and BPHYT_RS35715 are separated by 310 nucleotides BPHYT_RS35715 and BPHYT_RS35720 are separated by 87 nucleotides BPHYT_RS35720 and BPHYT_RS35725 are separated by 20 nucleotides
BPHYT_RS35705: BPHYT_RS35705 - FAD-dependent oxidoreductase, at 3,601,865 to 3,603,574
_RS35705
BPHYT_RS35710: BPHYT_RS35710 - hypothetical protein, at 3,603,640 to 3,603,876
_RS35710
BPHYT_RS35715: BPHYT_RS35715 - hypothetical protein, at 3,604,187 to 3,604,663
_RS35715
BPHYT_RS35720: BPHYT_RS35720 - phosphopantetheinyl transferase, at 3,604,751 to 3,605,446
_RS35720
BPHYT_RS35725: BPHYT_RS35725 - hypothetical protein, at 3,605,467 to 3,606,312
_RS35725
Position (kb)
3604
3605 Strain fitness (log2 ratio)
-1
0
1 at 3603.977 kb on + strand at 3604.027 kb on - strand at 3604.053 kb on + strand at 3604.205 kb on - strand at 3604.718 kb on + strand at 3604.755 kb on - strand at 3604.857 kb on + strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3605.055 kb on + strand, within BPHYT_RS35720 at 3605.055 kb on + strand, within BPHYT_RS35720 at 3605.419 kb on - strand at 3605.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 0.1 remove 3,603,977 + -0.8 3,604,027 - +0.1 3,604,053 + +0.3 3,604,205 - -0.3 3,604,718 + -0.2 3,604,755 - +0.2 3,604,857 + BPHYT_RS35720 0.15 -1.0 3,604,858 - BPHYT_RS35720 0.15 -1.4 3,604,858 - BPHYT_RS35720 0.15 +0.5 3,604,858 - BPHYT_RS35720 0.15 +0.6 3,604,858 - BPHYT_RS35720 0.15 -0.3 3,605,055 + BPHYT_RS35720 0.44 +0.2 3,605,055 + BPHYT_RS35720 0.44 +0.0 3,605,419 - -1.3 3,605,419 - -1.5
Or see this region's nucleotide sequence