Experiment: 24hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28300 and BPHYT_RS28305 are separated by 262 nucleotides BPHYT_RS28305 and BPHYT_RS28310 are separated by 226 nucleotides BPHYT_RS28310 and BPHYT_RS28315 are separated by 432 nucleotides
BPHYT_RS28300: BPHYT_RS28300 - histone nucleoid-structuring protein H-NS, at 1,904,600 to 1,904,902
_RS28300
BPHYT_RS28305: BPHYT_RS28305 - protein tyrosine phosphatase, at 1,905,165 to 1,905,686
_RS28305
BPHYT_RS28310: BPHYT_RS28310 - hypothetical protein, at 1,905,913 to 1,906,113
_RS28310
BPHYT_RS28315: BPHYT_RS28315 - transporter, at 1,906,546 to 1,907,223
_RS28315
Position (kb)
1905
1906 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1904.175 kb on - strand at 1904.205 kb on - strand at 1904.420 kb on + strand at 1904.494 kb on + strand at 1904.535 kb on - strand at 1904.722 kb on + strand, within BPHYT_RS28300 at 1904.910 kb on + strand at 1904.990 kb on + strand at 1904.990 kb on + strand at 1904.990 kb on + strand at 1904.991 kb on - strand at 1904.991 kb on - strand at 1904.991 kb on - strand at 1904.991 kb on - strand at 1905.397 kb on - strand, within BPHYT_RS28305 at 1905.763 kb on + strand at 1905.851 kb on - strand at 1905.878 kb on - strand at 1906.194 kb on + strand at 1906.250 kb on - strand at 1906.286 kb on + strand at 1906.299 kb on + strand at 1906.299 kb on + strand at 1906.305 kb on + strand at 1906.305 kb on + strand at 1906.305 kb on + strand at 1906.306 kb on - strand at 1906.429 kb on + strand at 1906.472 kb on - strand at 1906.488 kb on + strand at 1906.565 kb on + strand at 1906.580 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 0.1 remove 1,904,175 - +0.5 1,904,205 - -1.3 1,904,420 + -0.5 1,904,494 + +0.8 1,904,535 - +2.5 1,904,722 + BPHYT_RS28300 0.40 -1.9 1,904,910 + +0.0 1,904,990 + -0.7 1,904,990 + +1.5 1,904,990 + +0.6 1,904,991 - +0.8 1,904,991 - +0.4 1,904,991 - -1.4 1,904,991 - -0.7 1,905,397 - BPHYT_RS28305 0.44 -0.5 1,905,763 + -0.1 1,905,851 - -1.8 1,905,878 - -0.6 1,906,194 + +0.2 1,906,250 - +1.9 1,906,286 + +0.2 1,906,299 + +1.9 1,906,299 + +0.2 1,906,305 + +0.4 1,906,305 + +1.8 1,906,305 + -1.2 1,906,306 - -0.2 1,906,429 + -0.8 1,906,472 - -0.6 1,906,488 + -0.4 1,906,565 + +0.0 1,906,580 - +1.2
Or see this region's nucleotide sequence