Experiment: 24hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23060 and BPHYT_RS23065 are separated by 122 nucleotides BPHYT_RS23065 and BPHYT_RS23070 are separated by 40 nucleotides
BPHYT_RS23060: BPHYT_RS23060 - 3-isopropylmalate dehydratase large subunit, at 734,683 to 736,650
_RS23060
BPHYT_RS23065: BPHYT_RS23065 - MFS transporter, at 736,773 to 738,239
_RS23065
BPHYT_RS23070: BPHYT_RS23070 - hypothetical protein, at 738,280 to 740,211
_RS23070
Position (kb) 736
737
738
739 Strain fitness (log2 ratio) -1
0
1
2
3 at 735.800 kb on - strand, within BPHYT_RS23060 at 736.130 kb on - strand, within BPHYT_RS23060 at 736.332 kb on + strand, within BPHYT_RS23060 at 736.332 kb on + strand, within BPHYT_RS23060 at 736.705 kb on - strand at 736.738 kb on + strand at 736.993 kb on - strand, within BPHYT_RS23065 at 737.102 kb on + strand, within BPHYT_RS23065 at 737.102 kb on + strand, within BPHYT_RS23065 at 737.163 kb on + strand, within BPHYT_RS23065 at 737.790 kb on + strand, within BPHYT_RS23065 at 737.893 kb on + strand, within BPHYT_RS23065 at 737.897 kb on + strand, within BPHYT_RS23065 at 738.237 kb on + strand at 738.271 kb on + strand at 738.303 kb on + strand at 738.304 kb on - strand at 738.304 kb on - strand at 738.652 kb on - strand, within BPHYT_RS23070 at 738.806 kb on + strand, within BPHYT_RS23070 at 738.806 kb on + strand, within BPHYT_RS23070 at 738.807 kb on - strand, within BPHYT_RS23070 at 739.050 kb on + strand, within BPHYT_RS23070
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 0.1 remove 735,800 - BPHYT_RS23060 0.57 +2.5 736,130 - BPHYT_RS23060 0.74 +0.1 736,332 + BPHYT_RS23060 0.84 +0.2 736,332 + BPHYT_RS23060 0.84 -0.3 736,705 - -0.5 736,738 + +0.2 736,993 - BPHYT_RS23065 0.15 -1.5 737,102 + BPHYT_RS23065 0.22 +2.2 737,102 + BPHYT_RS23065 0.22 +1.8 737,163 + BPHYT_RS23065 0.27 -0.2 737,790 + BPHYT_RS23065 0.69 -0.1 737,893 + BPHYT_RS23065 0.76 +1.3 737,897 + BPHYT_RS23065 0.77 -1.0 738,237 + +0.2 738,271 + -0.2 738,303 + +0.5 738,304 - +3.5 738,304 - +0.9 738,652 - BPHYT_RS23070 0.19 -1.1 738,806 + BPHYT_RS23070 0.27 -0.7 738,806 + BPHYT_RS23070 0.27 +1.5 738,807 - BPHYT_RS23070 0.27 -1.2 739,050 + BPHYT_RS23070 0.40 -1.6
Or see this region's nucleotide sequence