Experiment: 24hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18385 and BPHYT_RS18390 are separated by 203 nucleotides
BPHYT_RS18385: BPHYT_RS18385 - 4-hydroxyphenylpyruvate dioxygenase, at 4,134,513 to 4,135,265
_RS18385
BPHYT_RS18390: BPHYT_RS18390 - membrane protein, at 4,135,469 to 4,136,401
_RS18390
Position (kb) 4135
4136
4137 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4134.478 kb on - strand at 4134.488 kb on + strand at 4134.489 kb on - strand at 4134.489 kb on - strand at 4134.505 kb on - strand at 4134.571 kb on - strand at 4134.703 kb on + strand, within BPHYT_RS18385 at 4134.703 kb on + strand, within BPHYT_RS18385 at 4134.757 kb on + strand, within BPHYT_RS18385 at 4134.757 kb on + strand, within BPHYT_RS18385 at 4134.758 kb on - strand, within BPHYT_RS18385 at 4134.758 kb on - strand, within BPHYT_RS18385 at 4134.928 kb on + strand, within BPHYT_RS18385 at 4135.039 kb on + strand, within BPHYT_RS18385 at 4135.039 kb on + strand, within BPHYT_RS18385 at 4135.338 kb on - strand at 4135.818 kb on + strand, within BPHYT_RS18390 at 4135.907 kb on + strand, within BPHYT_RS18390 at 4135.907 kb on + strand, within BPHYT_RS18390 at 4135.908 kb on - strand, within BPHYT_RS18390 at 4135.908 kb on - strand, within BPHYT_RS18390 at 4135.944 kb on - strand, within BPHYT_RS18390 at 4136.016 kb on + strand, within BPHYT_RS18390 at 4136.076 kb on + strand, within BPHYT_RS18390 at 4136.145 kb on - strand, within BPHYT_RS18390 at 4136.145 kb on - strand at 4136.175 kb on - strand, within BPHYT_RS18390 at 4136.340 kb on + strand at 4136.341 kb on - strand at 4136.341 kb on - strand at 4136.341 kb on - strand at 4136.341 kb on - strand at 4136.524 kb on + strand at 4136.622 kb on + strand at 4136.622 kb on + strand at 4136.622 kb on + strand at 4136.623 kb on - strand at 4136.698 kb on + strand at 4136.699 kb on - strand at 4136.874 kb on + strand at 4136.874 kb on + strand at 4136.875 kb on - strand at 4136.967 kb on + strand at 4136.968 kb on - strand at 4136.985 kb on + strand at 4137.028 kb on - strand at 4137.031 kb on + strand at 4137.031 kb on + strand at 4137.072 kb on + strand at 4137.072 kb on + strand at 4137.104 kb on + strand at 4137.104 kb on + strand at 4137.189 kb on - strand at 4137.253 kb on + strand at 4137.308 kb on + strand at 4137.340 kb on + strand at 4137.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 0.1 remove 4,134,478 - -0.3 4,134,488 + +0.3 4,134,489 - +0.4 4,134,489 - +0.4 4,134,505 - -0.9 4,134,571 - -0.2 4,134,703 + BPHYT_RS18385 0.25 +0.2 4,134,703 + BPHYT_RS18385 0.25 +0.3 4,134,757 + BPHYT_RS18385 0.32 +0.8 4,134,757 + BPHYT_RS18385 0.32 -1.0 4,134,758 - BPHYT_RS18385 0.33 +1.4 4,134,758 - BPHYT_RS18385 0.33 +1.1 4,134,928 + BPHYT_RS18385 0.55 +0.6 4,135,039 + BPHYT_RS18385 0.70 +0.9 4,135,039 + BPHYT_RS18385 0.70 +0.5 4,135,338 - +0.3 4,135,818 + BPHYT_RS18390 0.37 -1.9 4,135,907 + BPHYT_RS18390 0.47 -0.0 4,135,907 + BPHYT_RS18390 0.47 -1.0 4,135,908 - BPHYT_RS18390 0.47 -1.4 4,135,908 - BPHYT_RS18390 0.47 +0.0 4,135,944 - BPHYT_RS18390 0.51 -1.4 4,136,016 + BPHYT_RS18390 0.59 +0.1 4,136,076 + BPHYT_RS18390 0.65 +1.4 4,136,145 - BPHYT_RS18390 0.72 -0.4 4,136,145 - +1.4 4,136,175 - BPHYT_RS18390 0.76 +0.2 4,136,340 + +0.1 4,136,341 - +0.1 4,136,341 - +0.2 4,136,341 - +0.6 4,136,341 - -0.1 4,136,524 + +1.8 4,136,622 + -1.6 4,136,622 + -0.9 4,136,622 + -0.3 4,136,623 - -0.5 4,136,698 + -0.5 4,136,699 - +0.1 4,136,874 + -0.2 4,136,874 + +0.1 4,136,875 - -0.5 4,136,967 + +0.5 4,136,968 - -0.5 4,136,985 + -0.6 4,137,028 - +0.3 4,137,031 + +2.4 4,137,031 + -0.5 4,137,072 + -0.6 4,137,072 + +0.5 4,137,104 + -0.1 4,137,104 + -0.5 4,137,189 - +1.0 4,137,253 + -0.1 4,137,308 + -0.0 4,137,340 + -1.0 4,137,398 + -0.3
Or see this region's nucleotide sequence