Experiment: 24hr PHYTO4 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16995 and BPHYT_RS17000 are separated by 10 nucleotides BPHYT_RS17000 and BPHYT_RS17005 are separated by 194 nucleotides BPHYT_RS17005 and BPHYT_RS17010 are separated by 67 nucleotides BPHYT_RS17010 and BPHYT_RS17015 are separated by 130 nucleotides
BPHYT_RS16995: BPHYT_RS16995 - UDP-N-acetylmuramate--alanine ligase, at 3,854,687 to 3,855,340
_RS16995
BPHYT_RS17000: BPHYT_RS17000 - redoxin, at 3,855,351 to 3,855,902
_RS17000
BPHYT_RS17005: BPHYT_RS17005 - 3-dehydroquinate dehydratase, at 3,856,097 to 3,856,552
_RS17005
BPHYT_RS17010: BPHYT_RS17010 - acetyl-CoA carboxylase, at 3,856,620 to 3,857,090
_RS17010
BPHYT_RS17015: BPHYT_RS17015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 3,857,221 to 3,858,588
_RS17015
Position (kb)
3856
3857 Strain fitness (log2 ratio)
-1
0
1
2 at 3855.099 kb on - strand, within BPHYT_RS16995 at 3855.099 kb on - strand, within BPHYT_RS16995 at 3855.138 kb on - strand, within BPHYT_RS16995 at 3855.218 kb on + strand, within BPHYT_RS16995 at 3855.328 kb on + strand at 3855.329 kb on - strand at 3855.329 kb on - strand at 3855.329 kb on - strand at 3855.339 kb on - strand at 3855.339 kb on - strand at 3855.339 kb on - strand at 3855.356 kb on + strand at 3855.357 kb on - strand at 3855.741 kb on + strand, within BPHYT_RS17000
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 0.1 remove 3,855,099 - BPHYT_RS16995 0.63 +0.7 3,855,099 - BPHYT_RS16995 0.63 -0.1 3,855,138 - BPHYT_RS16995 0.69 +1.6 3,855,218 + BPHYT_RS16995 0.81 -0.5 3,855,328 + +0.4 3,855,329 - +0.2 3,855,329 - +0.4 3,855,329 - +0.6 3,855,339 - -0.3 3,855,339 - +0.8 3,855,339 - -0.2 3,855,356 + +1.0 3,855,357 - +2.3 3,855,741 + BPHYT_RS17000 0.71 +1.1
Or see this region's nucleotide sequence