Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36025 and BPHYT_RS36030 are separated by 50 nucleotides BPHYT_RS36030 and BPHYT_RS36035 are separated by 44 nucleotides BPHYT_RS36035 and BPHYT_RS36040 are separated by 24 nucleotides BPHYT_RS36040 and BPHYT_RS36045 overlap by 4 nucleotides BPHYT_RS36045 and BPHYT_RS36050 are separated by 20 nucleotides BPHYT_RS36050 and BPHYT_RS36055 are separated by 8 nucleotides
BPHYT_RS36025: BPHYT_RS36025 - hypothetical protein, at 34,722 to 35,090
_RS36025
BPHYT_RS36030: BPHYT_RS36030 - hypothetical protein, at 35,141 to 35,560
_RS36030
BPHYT_RS36035: BPHYT_RS36035 - hypothetical protein, at 35,605 to 36,003
_RS36035
BPHYT_RS36040: BPHYT_RS36040 - hypothetical protein, at 36,028 to 36,657
_RS36040
BPHYT_RS36045: BPHYT_RS36045 - hypothetical protein, at 36,654 to 36,911
_RS36045
BPHYT_RS36050: BPHYT_RS36050 - hypothetical protein, at 36,932 to 37,393
_RS36050
BPHYT_RS36055: BPHYT_RS36055 - hypothetical protein, at 37,402 to 37,695
_RS36055
Position (kb) 36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 35.362 kb on - strand, within BPHYT_RS36030 at 35.418 kb on - strand, within BPHYT_RS36030 at 35.692 kb on - strand, within BPHYT_RS36035 at 35.789 kb on + strand, within BPHYT_RS36035 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040 at 37.058 kb on - strand, within BPHYT_RS36050 at 37.095 kb on + strand, within BPHYT_RS36050 at 37.096 kb on - strand, within BPHYT_RS36050 at 37.190 kb on - strand, within BPHYT_RS36050 at 37.291 kb on + strand, within BPHYT_RS36050 at 37.393 kb on + strand at 37.393 kb on + strand at 37.394 kb on - strand at 37.437 kb on + strand, within BPHYT_RS36055
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 35,362 - BPHYT_RS36030 0.53 +0.0 35,418 - BPHYT_RS36030 0.66 -0.2 35,692 - BPHYT_RS36035 0.22 -3.0 35,789 + BPHYT_RS36035 0.46 +0.2 36,250 + BPHYT_RS36040 0.35 +3.3 36,250 + BPHYT_RS36040 0.35 -0.4 36,250 + BPHYT_RS36040 0.35 +0.3 36,250 + BPHYT_RS36040 0.35 -2.5 37,058 - BPHYT_RS36050 0.27 +0.8 37,095 + BPHYT_RS36050 0.35 -0.5 37,096 - BPHYT_RS36050 0.35 -1.7 37,190 - BPHYT_RS36050 0.56 -1.6 37,291 + BPHYT_RS36050 0.78 -0.9 37,393 + +2.3 37,393 + -1.4 37,394 - +0.1 37,437 + BPHYT_RS36055 0.12 +0.0
Or see this region's nucleotide sequence