Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35295 and BPHYT_RS35300 are separated by 186 nucleotides BPHYT_RS35300 and BPHYT_RS35305 are separated by 24 nucleotides BPHYT_RS35305 and BPHYT_RS35310 are separated by 52 nucleotides
BPHYT_RS35295: BPHYT_RS35295 - 3'-kinase, at 3,518,889 to 3,519,692
_RS35295
BPHYT_RS35300: BPHYT_RS35300 - flavin reductase, at 3,519,879 to 3,520,463
_RS35300
BPHYT_RS35305: BPHYT_RS35305 - low molecular weight phosphotyrosine protein phosphatase, at 3,520,488 to 3,520,817
_RS35305
BPHYT_RS35310: BPHYT_RS35310 - GCN5 family N-acetyltransferase, at 3,520,870 to 3,521,355
_RS35310
Position (kb)
3519
3520
3521 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3518.882 kb on + strand at 3519.136 kb on + strand, within BPHYT_RS35295 at 3519.680 kb on - strand at 3519.715 kb on + strand at 3519.716 kb on - strand at 3519.875 kb on - strand at 3519.875 kb on - strand at 3520.314 kb on + strand, within BPHYT_RS35300 at 3520.386 kb on + strand, within BPHYT_RS35300 at 3520.387 kb on - strand, within BPHYT_RS35300 at 3520.489 kb on + strand at 3520.490 kb on - strand at 3520.490 kb on - strand at 3520.490 kb on - strand at 3520.558 kb on + strand, within BPHYT_RS35305 at 3520.581 kb on + strand, within BPHYT_RS35305 at 3520.581 kb on + strand, within BPHYT_RS35305 at 3520.812 kb on + strand at 3520.961 kb on + strand, within BPHYT_RS35310 at 3520.962 kb on - strand, within BPHYT_RS35310 at 3521.111 kb on + strand, within BPHYT_RS35310 at 3521.429 kb on - strand at 3521.429 kb on - strand at 3521.436 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 3,518,882 + -0.8 3,519,136 + BPHYT_RS35295 0.31 +1.8 3,519,680 - +0.9 3,519,715 + +2.1 3,519,716 - -0.5 3,519,875 - -1.0 3,519,875 - +0.5 3,520,314 + BPHYT_RS35300 0.74 -1.0 3,520,386 + BPHYT_RS35300 0.87 -2.5 3,520,387 - BPHYT_RS35300 0.87 -2.3 3,520,489 + +0.4 3,520,490 - -1.1 3,520,490 - -1.0 3,520,490 - +0.2 3,520,558 + BPHYT_RS35305 0.21 +0.6 3,520,581 + BPHYT_RS35305 0.28 +0.1 3,520,581 + BPHYT_RS35305 0.28 +0.2 3,520,812 + -0.1 3,520,961 + BPHYT_RS35310 0.19 +0.9 3,520,962 - BPHYT_RS35310 0.19 +2.3 3,521,111 + BPHYT_RS35310 0.50 -1.5 3,521,429 - +1.3 3,521,429 - +0.6 3,521,436 - -0.3
Or see this region's nucleotide sequence