Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35230

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35225 and BPHYT_RS35230 are separated by 36 nucleotidesBPHYT_RS35230 and BPHYT_RS35235 are separated by 183 nucleotidesBPHYT_RS35235 and BPHYT_RS35240 are separated by 57 nucleotides BPHYT_RS35225: BPHYT_RS35225 - RNA polymerase subunit sigma-24, at 3,506,519 to 3,507,793 _RS35225 BPHYT_RS35230: BPHYT_RS35230 - type 11 methyltransferase, at 3,507,830 to 3,508,519 _RS35230 BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320 _RS35235 BPHYT_RS35240: BPHYT_RS35240 - membrane protein, at 3,509,378 to 3,509,956 _RS35240 Position (kb) 3507 3508 3509Strain fitness (log2 ratio) -1 0 1 2 3at 3506.861 kb on - strand, within BPHYT_RS35225at 3507.119 kb on + strand, within BPHYT_RS35225at 3507.141 kb on - strand, within BPHYT_RS35225at 3507.641 kb on + strand, within BPHYT_RS35225at 3507.641 kb on + strand, within BPHYT_RS35225at 3507.661 kb on - strand, within BPHYT_RS35225at 3507.987 kb on + strand, within BPHYT_RS35230at 3508.253 kb on - strand, within BPHYT_RS35230at 3508.253 kb on - strandat 3508.579 kb on + strandat 3508.628 kb on - strandat 3508.698 kb on - strandat 3508.783 kb on - strand, within BPHYT_RS35235at 3509.141 kb on + strand, within BPHYT_RS35235at 3509.325 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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3,506,861 - BPHYT_RS35225 0.27 +0.4
3,507,119 + BPHYT_RS35225 0.47 +0.1
3,507,141 - BPHYT_RS35225 0.49 -0.1
3,507,641 + BPHYT_RS35225 0.88 +0.2
3,507,641 + BPHYT_RS35225 0.88 -0.1
3,507,661 - BPHYT_RS35225 0.90 +0.6
3,507,987 + BPHYT_RS35230 0.23 -0.9
3,508,253 - BPHYT_RS35230 0.61 -0.9
3,508,253 - +2.9
3,508,579 + -1.0
3,508,628 - +0.9
3,508,698 - -1.7
3,508,783 - BPHYT_RS35235 0.13 -1.2
3,509,141 + BPHYT_RS35235 0.71 -1.7
3,509,325 + +0.4

Or see this region's nucleotide sequence