Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33150

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33140 and BPHYT_RS33150 are separated by 778 nucleotidesBPHYT_RS33150 and BPHYT_RS33155 are separated by 467 nucleotides BPHYT_RS33140: BPHYT_RS33140 - short-chain dehydrogenase, at 3,012,557 to 3,013,306 _RS33140 BPHYT_RS33150: BPHYT_RS33150 - beta-lactamase, at 3,014,085 to 3,015,017 _RS33150 BPHYT_RS33155: BPHYT_RS33155 - hypothetical protein, at 3,015,485 to 3,015,670 _RS33155 Position (kb) 3014 3015 3016Strain fitness (log2 ratio) -2 -1 0 1 2at 3013.319 kb on + strandat 3013.427 kb on + strandat 3013.590 kb on - strandat 3014.056 kb on + strandat 3014.855 kb on + strand, within BPHYT_RS33150at 3015.038 kb on - strandat 3015.142 kb on + strandat 3015.252 kb on - strandat 3015.426 kb on + strandat 3015.427 kb on - strandat 3015.530 kb on - strand, within BPHYT_RS33155at 3015.575 kb on - strand, within BPHYT_RS33155at 3015.636 kb on + strand, within BPHYT_RS33155at 3015.773 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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3,013,319 + -2.2
3,013,427 + -1.1
3,013,590 - +0.6
3,014,056 + +1.6
3,014,855 + BPHYT_RS33150 0.83 -1.1
3,015,038 - -1.0
3,015,142 + +0.1
3,015,252 - -0.5
3,015,426 + -1.0
3,015,427 - -0.5
3,015,530 - BPHYT_RS33155 0.24 -0.3
3,015,575 - BPHYT_RS33155 0.48 +1.9
3,015,636 + BPHYT_RS33155 0.81 +1.3
3,015,773 + -0.5

Or see this region's nucleotide sequence