Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32585

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32570 and BPHYT_RS32575 are separated by 314 nucleotidesBPHYT_RS32575 and BPHYT_RS32580 are separated by 68 nucleotidesBPHYT_RS32580 and BPHYT_RS32585 are separated by 17 nucleotidesBPHYT_RS32585 and BPHYT_RS32590 are separated by 800 nucleotides BPHYT_RS32570: BPHYT_RS32570 - 23S ribosomal RNA, at 2,876,210 to 2,879,112 _RS32570 BPHYT_RS32575: BPHYT_RS32575 - tRNA-Ala, at 2,879,427 to 2,879,502 _RS32575 BPHYT_RS32580: BPHYT_RS32580 - tRNA-Ile, at 2,879,571 to 2,879,647 _RS32580 BPHYT_RS32585: BPHYT_RS32585 - 16S ribosomal RNA, at 2,879,665 to 2,881,259 _RS32585 BPHYT_RS32590: BPHYT_RS32590 - RNA polymerase sigma factor RpoD, at 2,882,060 to 2,884,057 _RS32590 Position (kb) 2879 2880 2881 2882Strain fitness (log2 ratio) -2 -1 0 1 2at 2881.664 kb on + strandat 2881.665 kb on - strandat 2881.666 kb on + strandat 2881.666 kb on + strandat 2881.667 kb on - strandat 2881.668 kb on + strandat 2881.668 kb on + strandat 2881.668 kb on + strandat 2881.669 kb on - strandat 2881.669 kb on - strandat 2881.669 kb on - strandat 2881.669 kb on - strandat 2881.670 kb on + strandat 2881.670 kb on + strandat 2881.670 kb on + strandat 2881.671 kb on - strandat 2881.671 kb on - strandat 2881.671 kb on - strandat 2881.671 kb on - strandat 2881.671 kb on - strandat 2881.723 kb on + strandat 2881.724 kb on - strandat 2881.724 kb on - strandat 2881.726 kb on - strandat 2881.762 kb on + strandat 2881.763 kb on - strandat 2881.763 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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2,881,664 + +0.6
2,881,665 - +0.1
2,881,666 + +0.1
2,881,666 + -1.2
2,881,667 - +0.6
2,881,668 + -0.6
2,881,668 + -0.5
2,881,668 + -0.3
2,881,669 - -0.4
2,881,669 - -1.6
2,881,669 - -0.1
2,881,669 - -0.2
2,881,670 + -0.5
2,881,670 + +0.2
2,881,670 + -1.4
2,881,671 - -1.2
2,881,671 - +0.3
2,881,671 - +0.1
2,881,671 - +0.0
2,881,671 - +0.3
2,881,723 + +1.9
2,881,724 - -0.7
2,881,724 - +1.0
2,881,726 - +0.4
2,881,762 + -0.1
2,881,763 - -2.6
2,881,763 - +0.2

Or see this region's nucleotide sequence