Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32280 and BPHYT_RS32285 are separated by 441 nucleotides BPHYT_RS32285 and BPHYT_RS32290 are separated by 106 nucleotides BPHYT_RS32290 and BPHYT_RS32295 are separated by 86 nucleotides
BPHYT_RS32280: BPHYT_RS32280 - membrane protein, at 2,812,045 to 2,812,782
_RS32280
BPHYT_RS32285: BPHYT_RS32285 - aromatic amino acid aminotransferase, at 2,813,224 to 2,814,414
_RS32285
BPHYT_RS32290: BPHYT_RS32290 - hypothetical protein, at 2,814,521 to 2,815,210
_RS32290
BPHYT_RS32295: BPHYT_RS32295 - ATP-binding protein, at 2,815,297 to 2,816,085
_RS32295
Position (kb)
2813
2814
2815 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2812.427 kb on - strand, within BPHYT_RS32280 at 2812.570 kb on - strand, within BPHYT_RS32280 at 2813.009 kb on + strand at 2813.016 kb on - strand at 2813.400 kb on - strand, within BPHYT_RS32285 at 2813.969 kb on + strand, within BPHYT_RS32285 at 2813.970 kb on - strand, within BPHYT_RS32285 at 2814.071 kb on - strand, within BPHYT_RS32285 at 2814.118 kb on - strand, within BPHYT_RS32285 at 2814.193 kb on - strand, within BPHYT_RS32285 at 2814.198 kb on + strand, within BPHYT_RS32285 at 2814.199 kb on - strand, within BPHYT_RS32285 at 2814.308 kb on + strand at 2814.535 kb on - strand at 2814.846 kb on + strand, within BPHYT_RS32290 at 2814.857 kb on + strand, within BPHYT_RS32290 at 2815.094 kb on + strand, within BPHYT_RS32290
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 2,812,427 - BPHYT_RS32280 0.52 -1.2 2,812,570 - BPHYT_RS32280 0.71 -0.7 2,813,009 + -0.5 2,813,016 - +0.9 2,813,400 - BPHYT_RS32285 0.15 +0.9 2,813,969 + BPHYT_RS32285 0.63 -0.2 2,813,970 - BPHYT_RS32285 0.63 -0.5 2,814,071 - BPHYT_RS32285 0.71 +0.1 2,814,118 - BPHYT_RS32285 0.75 +1.2 2,814,193 - BPHYT_RS32285 0.81 -1.7 2,814,198 + BPHYT_RS32285 0.82 +0.1 2,814,199 - BPHYT_RS32285 0.82 +0.1 2,814,308 + -1.8 2,814,535 - +0.3 2,814,846 + BPHYT_RS32290 0.47 -0.8 2,814,857 + BPHYT_RS32290 0.49 -2.3 2,815,094 + BPHYT_RS32290 0.83 +2.1
Or see this region's nucleotide sequence