Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28750

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28740 and BPHYT_RS28745 are separated by 13 nucleotidesBPHYT_RS28745 and BPHYT_RS28750 are separated by 65 nucleotidesBPHYT_RS28750 and BPHYT_RS28755 are separated by 86 nucleotides BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326 _RS28740 BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149 _RS28745 BPHYT_RS28750: BPHYT_RS28750 - IclR family transcriptional regulator, at 2,003,215 to 2,003,499 _RS28750 BPHYT_RS28755: BPHYT_RS28755 - 3-(2-hydroxyphenyl) propionic acid transporter, at 2,003,586 to 2,005,019 _RS28755 Position (kb) 2003 2004Strain fitness (log2 ratio) -1 0 1 2at 2002.428 kb on + strand, within BPHYT_RS28745at 2002.778 kb on - strand, within BPHYT_RS28745at 2002.782 kb on - strand, within BPHYT_RS28745at 2002.843 kb on + strand, within BPHYT_RS28745at 2003.426 kb on - strand, within BPHYT_RS28750at 2003.549 kb on + strandat 2003.549 kb on + strandat 2003.549 kb on + strandat 2003.549 kb on + strandat 2003.550 kb on - strandat 2003.553 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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2,002,428 + BPHYT_RS28745 0.11 +0.4
2,002,778 - BPHYT_RS28745 0.54 -0.9
2,002,782 - BPHYT_RS28745 0.55 -1.6
2,002,843 + BPHYT_RS28745 0.62 -1.5
2,003,426 - BPHYT_RS28750 0.74 +0.4
2,003,549 + -0.2
2,003,549 + +0.4
2,003,549 + -0.4
2,003,549 + +0.1
2,003,550 - +1.9
2,003,553 + -0.4

Or see this region's nucleotide sequence