Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27565

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27560 and BPHYT_RS27565 are separated by 296 nucleotidesBPHYT_RS27565 and BPHYT_RS27570 are separated by 638 nucleotides BPHYT_RS27560: BPHYT_RS27560 - histidine kinase, at 1,756,016 to 1,757,128 _RS27560 BPHYT_RS27565: BPHYT_RS27565 - hydrolase, at 1,757,425 to 1,757,904 _RS27565 BPHYT_RS27570: BPHYT_RS27570 - universal stress protein UspA, at 1,758,543 to 1,759,040 _RS27570 Position (kb) 1757 1758Strain fitness (log2 ratio) -1 0 1 2 3at 1757.430 kb on + strandat 1757.595 kb on + strand, within BPHYT_RS27565at 1757.596 kb on - strand, within BPHYT_RS27565at 1757.681 kb on - strand, within BPHYT_RS27565at 1757.795 kb on + strand, within BPHYT_RS27565at 1757.795 kb on + strand, within BPHYT_RS27565at 1757.795 kb on + strand, within BPHYT_RS27565at 1757.813 kb on + strand, within BPHYT_RS27565at 1758.310 kb on + strandat 1758.311 kb on - strandat 1758.627 kb on - strand, within BPHYT_RS27570at 1758.890 kb on - strand, within BPHYT_RS27570

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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1,757,430 + +1.3
1,757,595 + BPHYT_RS27565 0.35 -1.3
1,757,596 - BPHYT_RS27565 0.36 +0.4
1,757,681 - BPHYT_RS27565 0.53 +2.9
1,757,795 + BPHYT_RS27565 0.77 -0.7
1,757,795 + BPHYT_RS27565 0.77 -1.0
1,757,795 + BPHYT_RS27565 0.77 +0.1
1,757,813 + BPHYT_RS27565 0.81 +1.3
1,758,310 + +2.0
1,758,311 - -0.8
1,758,627 - BPHYT_RS27570 0.17 +1.8
1,758,890 - BPHYT_RS27570 0.70 +0.7

Or see this region's nucleotide sequence