Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27250

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27240 and BPHYT_RS27245 are separated by 330 nucleotidesBPHYT_RS27245 and BPHYT_RS27250 are separated by 154 nucleotidesBPHYT_RS27250 and BPHYT_RS27255 are separated by 102 nucleotidesBPHYT_RS27255 and BPHYT_RS27260 are separated by 274 nucleotides BPHYT_RS27240: BPHYT_RS27240 - N-ethylmaleimide reductase, at 1,687,049 to 1,688,152 _RS27240 BPHYT_RS27245: BPHYT_RS27245 - hypothetical protein, at 1,688,483 to 1,688,725 _RS27245 BPHYT_RS27250: BPHYT_RS27250 - NAD-dependent epimerase/dehydratase, at 1,688,880 to 1,689,518 _RS27250 BPHYT_RS27255: BPHYT_RS27255 - hypothetical protein, at 1,689,621 to 1,689,800 _RS27255 BPHYT_RS27260: BPHYT_RS27260 - membrane protein, at 1,690,075 to 1,690,605 _RS27260 Position (kb) 1688 1689 1690Strain fitness (log2 ratio) -2 -1 0 1at 1687.953 kb on + strand, within BPHYT_RS27240at 1688.115 kb on + strandat 1688.115 kb on + strandat 1688.315 kb on - strandat 1688.315 kb on - strandat 1688.356 kb on + strandat 1688.357 kb on - strandat 1688.424 kb on + strandat 1688.428 kb on + strandat 1689.145 kb on + strand, within BPHYT_RS27250at 1689.320 kb on + strand, within BPHYT_RS27250at 1689.838 kb on - strandat 1690.156 kb on + strand, within BPHYT_RS27260

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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1,687,953 + BPHYT_RS27240 0.82 +0.2
1,688,115 + +0.3
1,688,115 + +0.7
1,688,315 - +1.3
1,688,315 - -0.8
1,688,356 + +1.0
1,688,357 - +0.3
1,688,424 + +0.3
1,688,428 + -0.1
1,689,145 + BPHYT_RS27250 0.41 -1.8
1,689,320 + BPHYT_RS27250 0.69 -1.5
1,689,838 - -1.2
1,690,156 + BPHYT_RS27260 0.15 +0.5

Or see this region's nucleotide sequence