Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27000

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26995 and BPHYT_RS27000 are separated by 187 nucleotidesBPHYT_RS27000 and BPHYT_RS27005 are separated by 137 nucleotides BPHYT_RS26995: BPHYT_RS26995 - peptidase S10, at 1,633,451 to 1,635,223 _RS26995 BPHYT_RS27000: BPHYT_RS27000 - aspartate aminotransferase, at 1,635,411 to 1,636,589 _RS27000 BPHYT_RS27005: BPHYT_RS27005 - RNA polymerase sigma54 factor, at 1,636,727 to 1,638,208 _RS27005 Position (kb) 1635 1636 1637Strain fitness (log2 ratio) -2 -1 0 1at 1634.574 kb on + strand, within BPHYT_RS26995at 1634.575 kb on - strand, within BPHYT_RS26995at 1634.575 kb on - strand, within BPHYT_RS26995at 1635.066 kb on - strandat 1635.066 kb on - strandat 1635.067 kb on + strandat 1635.446 kb on + strandat 1635.970 kb on + strand, within BPHYT_RS27000at 1635.971 kb on - strand, within BPHYT_RS27000at 1635.993 kb on + strand, within BPHYT_RS27000at 1636.023 kb on - strand, within BPHYT_RS27000at 1636.213 kb on + strand, within BPHYT_RS27000at 1636.213 kb on + strand, within BPHYT_RS27000at 1636.214 kb on - strand, within BPHYT_RS27000at 1636.214 kb on - strand, within BPHYT_RS27000at 1636.396 kb on + strand, within BPHYT_RS27000at 1636.479 kb on + strandat 1636.480 kb on - strandat 1636.777 kb on - strandat 1636.992 kb on + strand, within BPHYT_RS27005at 1636.993 kb on - strand, within BPHYT_RS27005at 1637.248 kb on - strand, within BPHYT_RS27005at 1637.343 kb on + strand, within BPHYT_RS27005at 1637.343 kb on + strand, within BPHYT_RS27005at 1637.344 kb on - strand, within BPHYT_RS27005at 1637.344 kb on - strand, within BPHYT_RS27005

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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1,634,574 + BPHYT_RS26995 0.63 -0.9
1,634,575 - BPHYT_RS26995 0.63 +0.8
1,634,575 - BPHYT_RS26995 0.63 -0.5
1,635,066 - -2.0
1,635,066 - -1.5
1,635,067 + -0.8
1,635,446 + +0.4
1,635,970 + BPHYT_RS27000 0.47 +0.5
1,635,971 - BPHYT_RS27000 0.47 -1.3
1,635,993 + BPHYT_RS27000 0.49 -1.2
1,636,023 - BPHYT_RS27000 0.52 -0.2
1,636,213 + BPHYT_RS27000 0.68 -0.2
1,636,213 + BPHYT_RS27000 0.68 -0.0
1,636,214 - BPHYT_RS27000 0.68 +1.3
1,636,214 - BPHYT_RS27000 0.68 +0.1
1,636,396 + BPHYT_RS27000 0.84 +0.1
1,636,479 + +0.9
1,636,480 - -1.8
1,636,777 - -0.3
1,636,992 + BPHYT_RS27005 0.18 -0.3
1,636,993 - BPHYT_RS27005 0.18 -1.1
1,637,248 - BPHYT_RS27005 0.35 +0.4
1,637,343 + BPHYT_RS27005 0.42 -0.0
1,637,343 + BPHYT_RS27005 0.42 -0.5
1,637,344 - BPHYT_RS27005 0.42 -0.8
1,637,344 - BPHYT_RS27005 0.42 -0.3

Or see this region's nucleotide sequence