Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26895 and BPHYT_RS26900 are separated by 82 nucleotides BPHYT_RS26900 and BPHYT_RS26905 are separated by 214 nucleotides BPHYT_RS26905 and BPHYT_RS26910 are separated by 67 nucleotides
BPHYT_RS26895: BPHYT_RS26895 - nicotinate phosphoribosyltransferase, at 1,606,296 to 1,607,723
_RS26895
BPHYT_RS26900: BPHYT_RS26900 - isoprenylcysteine carboxyl methyltransferase, at 1,607,806 to 1,608,492
_RS26900
BPHYT_RS26905: BPHYT_RS26905 - pilus assembly protein CpaE, at 1,608,707 to 1,609,909
_RS26905
BPHYT_RS26910: BPHYT_RS26910 - hypothetical protein, at 1,609,977 to 1,610,264
_RS26910
Position (kb) 1608
1609
1610 Strain fitness (log2 ratio) -2
-1
0
1 at 1607.931 kb on - strand, within BPHYT_RS26900 at 1608.350 kb on + strand, within BPHYT_RS26900 at 1608.370 kb on - strand, within BPHYT_RS26900 at 1610.232 kb on + strand, within BPHYT_RS26910 at 1610.232 kb on + strand, within BPHYT_RS26910 at 1610.294 kb on - strand at 1610.696 kb on + strand at 1610.783 kb on - strand at 1610.783 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 1,607,931 - BPHYT_RS26900 0.18 -1.6 1,608,350 + BPHYT_RS26900 0.79 -0.4 1,608,370 - BPHYT_RS26900 0.82 -1.8 1,610,232 + BPHYT_RS26910 0.89 +1.3 1,610,232 + BPHYT_RS26910 0.89 +0.4 1,610,294 - +0.9 1,610,696 + -2.0 1,610,783 - -0.3 1,610,783 - +0.4
Or see this region's nucleotide sequence